Skip to main content
. 2019 Jun 19;9(16):4567–4579. doi: 10.7150/thno.31502

Figure 2.

Figure 2

Performance of different protocols measured using RT-qPCR. A, the number of TaqMan RT-qPCR replicates (from a total of 12) in which the studied miRNAs were detected. Twelve RT-qPCR reactions were performed for each method (3 EV isolations and miRNA extractions x 2 cDNA synthesis reactions x 2 technical duplicates of each cDNA reaction). B, relative quantification (in comparison with ath-miR-159a) of miR-21-5p, miR-451a, miR-92a-3p, and miR-22-3p and C, of miR-1911-5p, miR-1264, miR-30c-5p, and miR-34b-3p. Abbreviations: ultracentrifugation (UC), miRCURY Exosome Isolation Kit from Qiagen (QIA), Total Exosome Isolation Reagent from Invitrogen (INV), mirVana PARIS Kit from Ambion (PAR), and Plasma/Serum RNA Purification Kit from Norgen (NOR).