Abstract
Idiopathic and ischemic dilated cardiomyopathies (DCM) are the most common types of DCM, and both exhibit the same histopathological feature of fibrosis. Prolidase is an enzyme that serves a rate‐limiting function in collagen turnover. Several studies have shown increased prolidase activity in fibrosis, though controversy persists. In this study, we measured prolidase enzyme activity in patients with idiopathic or ischemic DCM and in healthy controls, making this, to our knowledge, the first study to do so. What we found is that serumprolidase activity was significantly lower in both DCM groups relative to healthy volunteers and lower in ischemic DCM than idiopathic. These intriguing results could be attributed either to decreased collagen turnover in the heart tissues in which DCM develops, a result of diminished functional heart tissue, or to decreased physical activity levels among DCM patients stemming from their heart failure. Either way, further studies are needed to verify and clarify our results. J. Clin. Lab. Anal. 24:213–218, 2010. © 2010 Wiley‐Liss, Inc.
Keywords: serum prolidase activity, idiopathic dilated cardiomyopathy, ischemic dilated cardiomyopathy
REFERENCES
- 1. Zipes OP, Libby P, Bonow RO, Braunwald E, editors. 2005. Braunwald's Heart Disease: A Textbook of Cardiovascular Medicine. Philadelphia: Elsevier, Inc. [Google Scholar]
- 2. Maron BJ, Towbin JA, Thiene G, et al. American Heart Association; Council on Clinical Cardiology, Heart Failure and Transplantation Committee; Quality of Care and Outcomes Research and Functional Genomics and Translational Biology Interdisciplinary Working Groups; Council on Epidemiology and Prevention. Contemporary definitions and classification of the cardiomyopathies: An American Heart Association Scientific Statement from the Council on Clinical Cardiology, Heart Failure and Transplantation Committee; Quality of Care and Outcomes Research and Functional Genomics and Translational Biology Interdisciplinary Working Groups; and Council on Epidemiology and Prevention. Circulation 2006;113:1807–1816. [DOI] [PubMed] [Google Scholar]
- 3. Rojkind M, Gatmaitan Z, Mackensen S, Giambrone MA, Ponce P, Reid LM. Connective tissue biomatrix: Its isolation and utilization for long‐term cultures of normal rat hepatocytes. J Cell Biol 1980;87:255–263. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 4. Myara I, Charpentier C, Lemonnier A. Optimal conditions for prolidase assay by praline colorimetric determination: Application to iminodipeptiduria. Clin Chim Acta 1982;125:193–205. [DOI] [PubMed] [Google Scholar]
- 5. Schuppan D, Schmid M, Somasundaram R, et al. Collagens in the liver extracellular matrix bind hepatocyte growth factor. Gastroenterology 1998;114:139–152. [DOI] [PubMed] [Google Scholar]
- 6. Brosset B, Myara I, Fabre M, Lemonnier A. Plasma prolidase and prolinase activity in alcoholic liver disease. Clin Chim Acta 1988;175:291–295. [DOI] [PubMed] [Google Scholar]
- 7. Abraham P, Wilfred G, Ramakrishna B. Plasma prolidase may be an index of liver fibrosis in the rat. Clin Chim Acta 2000;295:199–202. [DOI] [PubMed] [Google Scholar]
- 8. Tarcin O, Avsar K, Demirturk L, et al. In vivo inefficiency of pentoxifylline and interferon‐alpha on hepatic fibrosis in biliary‐obstructed rats: Assessment by tissue collagen content and prolidase activity. J Gastroenterol Hepatol 2003;18:437–444. [DOI] [PubMed] [Google Scholar]
- 9. Teichholz LE, Kreulen T, Herman MV, Gorlin R. Problems in echocardiographic volume determinations: Echocardiographic–angiographic correlations in the presence of absence of asynergy. Am J Cardiol 1976;37:7–11. [DOI] [PubMed] [Google Scholar]
- 10. Chinard FP. Photometric estimation of proline and ornithine. J Biol Chem 1952;199:91–95. [PubMed] [Google Scholar]
- 11. Yildiz A, Demirbag R, Yilmaz R, et al. The association of serum prolidase activity with the presence and severity of coronary artery disease. Coron Artery Dis 2008;19:319–325. [DOI] [PubMed] [Google Scholar]
- 12. Demirbag R, Yildiz A, Gur M, Yilmaz R, Elçi K, Aksoy N. Serum prolidase activity in patients with hypertension and its relation with left ventricular hypertrophy. Clin Biochem 2007;40:1020–1025. [DOI] [PubMed] [Google Scholar]
- 13. Camuzcuoglu H, Arioz DT, Toy H, Kurt S, Celik H, Aksoy N. Assessment of preoperative serum prolidase activity in epithelial ovarian cancer. Eur J Obstet Gynecol Reprod Biol 2009;147:97–100. [DOI] [PubMed] [Google Scholar]
- 14. Karna E, Surazynski A, Palka J. Collagen metabolism disturbances are accompanied by an increase in prolidase activity in lung carcinoma planoepitheliale. Int J Exp Pathol 2000;81:341–347. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 15. Wolañska M, Sobolewski K, Drozdzewicz M. Integrins and prolidase activity in uterine leiomyoma during tumor growth. Ginekol Pol 2001;72:121–126. [PubMed] [Google Scholar]
- 16. Palka JA, Phang JM. Prolidase activity in fibroblasts is regulated by interaction of extracellular matrix with cell surface integrin receptors. J Cell Biochem 1997;67:166–175. [DOI] [PubMed] [Google Scholar]
- 17. Kokturk A, Kaya TI, Ikizoglu G, Koca A. Prolidase deficiency. Int J Dermatol 2002;41:45–48. [DOI] [PubMed] [Google Scholar]
- 18. Falik‐Zaccai TC, Khayat M, Luder A, et al. Broad spectrum of developmental delay in a large cohort of prolidase deficiency patients demonstrates marked interfamilial and intrafamilial phenotypic variability. Am J Med Genet B Neuropsychiatr Genet 2010;153B:46–56. [DOI] [PubMed] [Google Scholar]
- 19. Leoni A, Cetta G, Tenni R, et al. Prolidase deficiency in two siblings with chronic leg ulcerations. Arch Dermatol 1987;123:493–499. [PubMed] [Google Scholar]
- 20. Cakmak A, Zeyrek D, Atas A, Celik H, Aksoy N, Erel O. Serum prolidase activity and oxidative status in patients with bronchial asthma. J Clin Lab Anal 2009;23:132–138. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 21. Myara I, Myara A, Mangeot M, Fabre M, Charpentier C, Lemonnier A. Plasma prolidase activity: A possible index of collagen catabolism in chronic liver disease. Clin Chem 1984;30:211–215. [PubMed] [Google Scholar]
- 22. Kurien BT, Patel NC, Porter AC, et al. Prolidase deficiency and the biochemical assays used in its diagnosis. Anal Biochem 2006;349:165–175. [DOI] [PubMed] [Google Scholar]
- 23. Langberg H, Skovgaard D, Asp S, Kjaer M. Time pattern of exercise‐induced changes in type I collagen turnover after prolonged endurance exercise in humans. Calcif Tissue Int 2000;67:41–44. [DOI] [PubMed] [Google Scholar]
- 24. Wallace JD, Cuneo RC, Lundberg PA, et al. Responses of markers of bone and collagen turnover to exercise, growth hormone (GH) administration, and GH withdrawal in trained adult males. J Clin Endocrinol Metab 2000;85:124–133. [DOI] [PubMed] [Google Scholar]