Skip to main content
PMC home page
Journal of Clinical Laboratory Analysis logoLink to Journal of Clinical Laboratory Analysis
. 2009 Sep 22;23(5):336–340. doi: 10.1002/jcla.20338

Evaluation of DPC immulite 2000® Toxoplasma quantitative IgG/IgM kits for automated toxoplasmosis serology with immulite 2000® †

Florence Robert‐Gangneux 1,2,✉, Chantal Bourhis 3, Sylviane Chevrier 1, Jean‐Pierre Gangneux 1,2
PMCID: PMC6648954  PMID: 19774623

Abstract

In this study, we evaluated a new immunoassay for the automated detection of anti‐toxoplasma IgG and IgM with Immulite 2000® (DPC‐Siemens, La Garenne‐Colombes, France). We tested 280 sera from 112 patients with past infection (PI), 40 PI with residual IgM, 75 seronegatives, 16 infants (31 sera) monitored for neonatal screening of congenital toxoplasmosis, and 13 patients with recent seroconversion (SC) (22 sequential sera). Detection sensitivity and specificity for IgG were 99 and 100%, respectively. IgG titers obtained with Immulite 2000® were higher than with Vidas® (BioMérieux, Marcy I'Etoile, France) and Access® (Beckman‐Coulter, Villepinte, France) (paired Wilcoxon test z=4.44 and z=3.67, respectively, P<0.001). IgM specificity was 100%. Detection sensitivity for IgM was 100% in the SC group, 86% in congenitally infected infants, and 75% in PI with persistent IgM. IgM detection seemed less prolonged in time than with the IgM Access® and ISAGA® IgM techniques. J. Clin. Lab. Anal. 23:336–340, 2009. © 2009 Wiley‐Liss, Inc.

Keywords: toxoplasmosis, serological diagnosis, ELISA, antibody, TORCH screening

†

Part of these data were presented at the Congrès du Syndicat National des Biologistes Hospitaliers (Saint‐Malo; September 2006).

REFERENCES

  • 1. Guerina NG, Hsu HW, Meissner HC, et al. Neonatal serologic screening and early treatment for congenital Toxoplasma gondii infection. The New England Regional Toxoplasma Working Group. N Engl J Med 1994;330:1858–1863. [DOI] [PubMed] [Google Scholar]
  • 2. Montoya JG, Liesenfeld O, Kinney S, Press C, Remington JS. VIDAS test for avidity of Toxoplasma‐specific immunoglobulin G for confirmatory testing of pregnant women. J Clin Microbiol 2002;40:2504–2508. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3. Roux‐Buisson N, Fricker‐Hidalgo H, Foussadier A, et al.. Comparative analysis of the VIDAS Toxo IgG IV assay in the detection of antibodies to Toxoplasma gondii . Diagn Microbiol Infect Dis 2005;53:79–81. [DOI] [PubMed] [Google Scholar]
  • 4. Foulon W, Villena I, Stray‐Pedersen B, et al. Treatment of toxoplasmosis during pregnancy: A multicenter study of impact on fetal transmission and children's sequelae at age 1 year. Am J Obstet Gynecol 1999;1802:410–415. [DOI] [PubMed] [Google Scholar]
  • 5. Fricker‐Hidalgo H, Pelloux H, Racinet C, Bost M, Goullier‐Fleuret A, Ambroise‐Thomas P. Congenital toxoplasmosis: Specific IgM in fetal blood, cord blood and in the newborn. Ann Biol Clin 1996;54:165–168. [PubMed] [Google Scholar]
  • 6. Hezard N, Marx‐Chemla C, Foudrinier F, et al. Prenatal diagnosis of congenital toxoplasmosis in 261 pregnancies. Prenat Diagn 1997;17:1047–1054. [DOI] [PubMed] [Google Scholar]
  • 7. Hohlfeld P, Daffos F, Costa JM, Thulliez P, Forestier F. Prenatal diagnosis of congenital toxoplasmosis with a polymerase chain reaction test on amniotic fluid. N Engl J Med 1994;331:695–699. [DOI] [PubMed] [Google Scholar]
  • 8. Pinon JM, Chemla C, Villena I, et al. Early neonatal diagnosis of congenital toxoplasmosis: Value of comparative enzyme‐linked immunofiltration assay immunological profiles and anti‐Toxoplasma gondii immunoglobulin M or IgA immunocapture and implications for postnatal therapeutic strategies. J Clin Microbiol 1996;34:579–583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9. Robert‐Gangneux F, Gavinet MF, Ancelle T, Raymond J, Tourte‐Schaefer C, Dupouy‐Camet J. Congenital toxoplasmosis: Value of prenatal diagnosis and early postnatal diagnosis. A retrospective study of 110 cases. J Clin Microbiol 1999;37:2893–2898. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10. Koppe JG, Loewer‐Sieger DH, de Roever‐Bonnet H. Results of 20 years follow‐up of congenital toxoplasmosis. Lancet 1986;i:254–256. [DOI] [PubMed] [Google Scholar]
  • 11. McAuley J, Boyer KM, Patel D, et al. Early and longitudinal evaluations of treated infants and children and untreated historical patients with congenital toxoplasmosis: The Chicago Collaborative Treatment Trial. Clin Infect Dis 1994;18:38–72. [DOI] [PubMed] [Google Scholar]
  • 12. Villena I, Aubert D, Leroux B, et al. Pyrimethamine‐sulfadoxine treatment of congenital toxoplasmosis: Follow‐up of 78 cases between 1980 and 1997. Reims Toxoplasmosis Group. Scand J Infect Dis 1998;30:295–300. [DOI] [PubMed] [Google Scholar]
  • 13. Owen WE, Martins TB, Litwin CM, Roberts WL. Performance characteristics of six Immulite 2000 TORCH assays. Am J Clin Pathol 2006;126:900–905. [DOI] [PubMed] [Google Scholar]
  • 14. Villena I, Bory JP, Chemla C, Hornoy P, Pinon JM. Congenital toxoplasmosis: Necessity of clinical and ultrasound follow‐up despite negative amniocentesis. Prenat Diagn 2003;23:1098–1099. [DOI] [PubMed] [Google Scholar]
  • 15. Kaul R, Chen P, Binder SR. Detection of immunoglobulin M antibodies specific for Toxoplasma gondii with increased selectivity for recently acquired infections. J Clin Microbiol 2004;42:5705–5709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16. Roberts F, Mc Leod R. Pathogenesis of toxoplasmic retinochorPIditis. Parasitol Today 1999;15:51–58. [DOI] [PubMed] [Google Scholar]
  • 17. Fricker‐Hidalgo H, Pelloux H, Racinet C, et al. Detection of Toxoplasma gondii in 94 placentae from infected women by polymerase chain reaction, in vivo, and in vitro cultures. Placenta 1998;19:545–549. [DOI] [PubMed] [Google Scholar]
  • 18. Robert‐Gangneux F, Commere V, Tourte‐Schaefer C, Dupouy‐Camet J. Performance of a Western blot assay to compare mother and newborn anti‐Toxoplasma antibodies for the early neonatal diagnosis of congenital toxoplasmosis. Eur J Clin Microbiol Infect Dis 1999;18:648–654. [DOI] [PubMed] [Google Scholar]
  • 19. Tissot Dupont D, Fricker‐Hidalgo H, Brenier‐Pinchart MP, Bost‐Bru C, Ambroise‐Thomas P, Pelloux H. Usefulness of Western blot in serological follow‐up of newborns suspected of congenital toxoplasmosis. Eur J Clin Microbiol Infect Dis 2003;22:122–125. [DOI] [PubMed] [Google Scholar]
  • 20. Pinon JM, Dumon H, Chemla C, et al. Strategy for diagnosis of congenital toxoplasmosis: Evaluation of methods comparing mothers and newborns and standard methods for postnatal detection of immunoglobulin G, M, and A antibodies. J Clin Microbiol 2001;39:2267–2271. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Laboratory Analysis are provided here courtesy of Wiley

RESOURCES