Figure 7. Activation of the NADPH oxidase of neutrophil-like cells by HOCl-treated serum albumin and immunoglobulin G occurs predominantly via a PI3K-dependent signaling pathway.

Effect of 10 μM diphenyleneiodonium (DPI; NADPH oxidase inhibitor), 100 nM wortmannin (PI3K inhibitor) and 200 nM Gö 6983 (protein kinase C (PKC) inhibitor) on the NADPH oxidase activation mediated by (a) 3 mg · mL⁻¹ HSA _50xHOCl, (b) 3 mg · mL⁻¹ IgG _150xHOCl and (c) 0.2 µM PMA was tested. (d) Results shown in a, b and c are expressed as integrated total counts (means and standard deviations of three independent measurements) higher than the respective buffer control. Student’s t-test: **p<0.01, ***p<0.001.

Figure 7—figure supplement 1. Addition of catalase, superoxide dismutase (SOD) or both inhibited immune cell-induced chemiluminescence of lucigenin.

(a) The addition of catalase (CAT), superoxide dismutase (SOD) or both to differentiated PLB-985 cells incubated with HOCl-treated HSA prevented lucigenin chemiluminescence. (b) Results shown in a are expressed as integrated total counts (means and standard deviations of three independent measurements) higher or lower than buffer control. Student’s t-test: ***p<0.001. PMA-induced activation of NADPH-oxidase was set to 100%.