Figure 7.

Enriched genes and pathways in islets from 15-wk-old lean and GDM offspring. (A) Heat map of 27 genes changed (>1.5-fold and FDR of <0.05) in the islets of GDM offspring compared with lean offspring. (B) Heat map of 24 genes involved in endocrine pancreas development and function. (C) Islet gene qPCR validation of RNA-seq expression profiles. qPCR data are mean ± SEM. The HFS diet included 45% fat (4.73 kcal/g); the LF diet included 10% fat (3.85 kcal/g). *P < 0.05, LF vs HFS; ^§P < 0.05, lean vs GDM using a two-way ANOVA followed by a Bonferroni posttest (n = 6 by group).