Fig. 2.

Cell Viability of (a). HUVEC (b). pericytes and (c). MDA-MB 231 cells when Treated with Propolis Extracts. The cells were treated with various concentrations of D01 and D02. After 48 hours of incubation, 10μl/well of the CCK-8 solution was added and the absorbance at 450nM was measured. Data are expressed as absorbance values with normalization to the basal values of cells with DMSO as the control, which show means ±SD and represent biological replicates. Taxol was used as a positive control of anti-angiogenesis to be compared with the samples. The best fit curve was produced by analyzing the predicted residual error sum of squares (PRESS). Bullets signify the actual average experimental data. The experiment was repeated three times with five technical replicates in each experiment. Data of D02 did not produce a converged curve on HUVECs' and pericytes' curves. Furthermore, D02 barely exhibited an inhibitory or cytotoxic effect on MDA-MB-231 cells (the plot is not depicted for efficiency).