Table 1. Mass transitions used for LC-MS/MS experiments.
| Nucleotides | Formula | Parent/Product (positive polarity) |
Comment (for 15N experiment) |
|---|---|---|---|
| AMP | C10H14N5O7P | 348/136 | Product has all N |
| 15N_AMP_1 | 349/137 | ||
| 15N_AMP_2 | 350/138 | ||
| 15N_AMP_3 | 351/139 | ||
| 15N_AMP_4 | 352/140 | ||
| 15N_AMP_5 | 353/141 | ||
| GMP | C10H14N5O8P | 364/152 | Product has all N |
| 15N_GMP_1 | 365/153 | ||
| 15N_GMP_2 | 366/154 | ||
| 15N_GMP_3 | 367/155 | ||
| 15N_GMP_4 | 368/156 | ||
| 15N_GMP_5 | 369/157 | ||
| CMP | C9H14N3O8P | 324/112 | Product has all N |
| 15N_CMP_1 | 325/113 | ||
| 15N_CMP_2 | 326/114 | ||
| 15N_CMP_3 | 327/115 | ||
| UMP | C9H13N2O9P | 325/113 | Product has all N |
| 15N_UMP_1 | 326/114 | ||
| 15N_UMP_2 | 327/115 | ||
| Trehalose and sugar phosphates | Formula | Parent/Product (negative polarity) |
Comment (for 13C experiment) |
| Trehalose | C12H22O11 | 341.3/179.3 | |
| 13C_Trehalose_12 | 353.3/185.3 | Product has 6 C all of which are labeled | |
| G3P | C3H7O6P | 169/97 | Monitoring the phosphate release |
| 13C_G3P_3 | 172/97 | ||
| 3 PG | C3H7O7P | 185/97 | Monitoring the phosphate release |
| 13C_3 PG_3 | 188/97 | ||
| G6P | C6H13O9P | 259/97 | Monitoring the phosphate release |
| 13C_G6P_6 | 265/97 | ||
| 6 PG | C6H13O10P | 275/97 | Monitoring the phosphate release |
| 13C_6 PG_6 | 281/97 | ||
| R5P | C5H11O8P | 229/97 | Monitoring the phosphate release |
| 13C_R5P_5 | 234/97 | ||
| S7P | C7H15O10P | 289/97 | Monitoring the phosphate release |
| 13C_S7P_5 | 294/97 |