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. 2019 Jul 26;6(4):ENEURO.0236-19.2019. doi: 10.1523/ENEURO.0236-19.2019

Figure 3.

Figure 3.

Ubiquitous loss of TRESK currents preferentially increases the excitability of small IB4− TG neurons. A, Representative traces of APs generated by incremental depolarizing current injections in TG neurons from WT and TRESK KO mice. The values of Vrest and the current amplitude are indicated. B, C, Mean rheobase (B; the minimum amount of current required to elicit at least 1 AP) and Rin (C) of subpopulations of TG neurons from TRESK WT, HET, and KO mice (n = 14–28 neurons in each group; for details of the intrinsic properties of TG neurons, see Table 1). *p < 0.05, **p < 0.01; one-way ANOVA with post hoc Bonferroni test compared with the corresponding WT group; #p < 0.05, ##p < 0.01; compared with the corresponding HET group. D–F, Input/output plots of the spike frequency in response to incremental depolarizing current injections in small IB4− (D), small IB4+ (E) and medium-sized (F) TG neurons from TRESK WT, HET, and KO mice (same neurons as in B). &p < 0.05; two-way RM ANOVA; TRESK KO neurons versus WT and HET groups. **p < 0.01, ***p < 0.001; one-way ANOVA with post hoc Bonferroni test compared with the corresponding WT group; ##p < 0.01; compared with the corresponding HET group. Inset, The slope (spikes/sec/nA) of the input/output relationships between 25 and 125 pA current injections. *p < 0.05, #p < 0.05; one-way ANOVA with post hoc Bonferroni test compared with the WT and HET groups, respectively. G, Mean rheobase of small IB4−, small IB4+, and medium-sized TG neurons from WT mice (same neurons as in B, WT groups). *p < 0.05, **p < 0.01, ***p < 0.001; one-way ANOVA with post hoc Bonferroni test. H, Input/output plots of the spike frequency in response to incremental depolarizing current injections in subpopulations of WT TG neurons (same WT neurons as in B WT groups). *p < 0.05, **p < 0.01, ***p < 0.001; two-way RM ANOVA. I, The plot of steady-state membrane potential versus injected current in medium-sized TG neurons from WT and KO mice (same neurons as in B, medium-sized groups).