(A-A’) DAPI (blue), anti-Dcp1 (red) and Annexin V (green) staining of scrib1 mutant tumours. (B-B’) DAPI (blue), anti-Dcp1 (red) and Annexin V (green) staining of egr3;scrib1 double mutant tumours. (C-C’’) scrib1 mutant tumour stained with DAPI (blue), anti-Dcp1 (green) and anti-Def (red) antibodies. D-D’’, Enlargement of inset from C’’ (white outline) showing anti-Dcp1 (D), anti-Def (D’) and merged (D’’) channels. E, F, Quantification of tumour volume (E) and tumour cell death (F) in wing imaginal discs from scrib1 (n = 19) and defsk3;scrib1 (n = 18) mutant larvae. G-H’, representative immunofluorescence images of tissues quantified in E, F. A, B, C, G, H, Scale bars = 50 μm; D, Scale bar = 10 μm. Statistical analysis: E, F, Mann-Whitney test, E, **p=0.0015, F, ***p<0.0001.