Table 2.
Virus abbreviation and straina | Plant tissue | Virus-specific reads (fraction of total) reads × 1e5)b | Contig length (nt) | Mean coverage (per nt position)c |
---|---|---|---|---|
BevuMVl-KWS2320d | roots | ≥5.1 | 2704 | ≥454 |
leaves | 1.0 | 2810 | 579 | |
inflorescences | ≥9.2 | 2825 | ≥1416 | |
seeds | ≥34 | 2799 | ≥357 | |
seedlings | 4.9 | 2800 | 410 | |
CasaMV1-MPC/MSUe | roots | 3.4 | 2818 | 137 |
stems | 12 | 2824 | 450 | |
leaves | 3.3 | 2805 | 177 | |
flower buds | 9.8 | 2823 | 353 | |
flowers | 10 | 2811 | 394 | |
DapiMV1-RioRiataf | stem | 5.5 | 2796 | 93 |
leaf | 4.9 | 2806 | 102 | |
flower bud | 6.7 | 2791 | 106 | |
PeexMV1-OPGC943g | apical shoot | 2.1 | 2659 | 43 |
trichome | 2.3 | 2680 | 37 | |
callus | 1.0 | 2667 | 14 | |
flowers | ≥30 | 2700 | ≥740 | |
seedling | 1.0 | 2664 | 17 | |
SochMV1-G4h | leaves | 0.8 | 2750 | 84 |
immature ovules | 5.5 | 2747 | 1326 | |
mature ovules | ≥6.5 | 2754 | ≥1465 |
The viruses shown here are those from Table 1 for which a complete coding sequence could be assembled from each of the different sampled tissues.
Each fractional value has been multiplied by 100,000 for ease of comparison, The ≥ symbol reflects that in some cases a single SRA data set scored ≥20,000 hits (the limit for reporting by the online version of Blast at NCBI).
Positional coverage determined after using the Map Reads to Contigs function in CLC Genomics Workbench.
The five BevuMV1-KWS2320 nt sequences are 100% identical across a 2680-nt region of shared overlap. SRA data sets for young leaves (BioProject PRJNA254489) and old leaves (BioProject PRJNA41497) (Stracke et al., 2014) were combined for this analysis.
The five CasaMV1-MPC/MSU nt sequences are >99.9% identical (1 nt difference) across a 2804-nt region of shared overlap. SRA data sets for young, mature, and mixed leaves were combined for this analysis. SRA data sets for primary stem and stem–petioles were also combined for this analysis.
The three DapiMV1-RioRiata nt sequences are 100% identical across a 2791-nt region of shared overlap.
The five PeexMV1-OPGC943 nt sequences are 100% identical across a 2665-nt region of shared overlap.
The three SochMV1-G4 nt sequences are 100% identical across a 2732-nt region of shared overlap.