In the article “Activity-Independent Regulation of Dendrite Patterning by Postsynaptic Density Protein PSD-95,” by Erik I. Charych, Barbara F. Akum, Joshua S. Goldberg, Rebecka J. Jörnsten, Christopher Rongo, James Q. Zheng, and Bonnie L. Firestein, which appeared on pages 10164–10176 of the October 4, 2006 issue, the micrograph representing a PSD-95-DsRed1-transfected neuron in Figure 2 A (right) was accidentally duplicated in Figure 6 A (left), which should have represented a GFP-transfected neuron. The micrograph in Figure 6 has been replaced with that representing a GFP-transfected neuron and is printed here.
Figure 6.
PSD-95 stops cypin-promoted dendritic branching. A, Hippocampal neurons were transfected with cDNAs encoding the indicated constructs on DIV 10. Scale bar, 10 μm. B, Dendrite number was assessed at 12 DIV. Overexpression of PSD-95-DsRed1 decreased cypin-promoted increases in primary and secondary dendrite number (cypin + PSD-95). PSD-95 decreased secondary dendrite number below basal (GFP) levels regardless of cypin overexpression. n values are as follows: GFP, 33; GFP + PSD-95-dsRed, 10; cypin, 30; cypin + PSD-95-dsRed, 20. *p < 0.05 and ***p < 0.001 by Kruskal–Wallis test followed by Dunn’s multiple-comparisons test. C, Left, Overexpression of PSD-95 decreases the proportion of primary dendrites that branch (ANODEV, p = 0.0003). PSD-95 blocks the effect of cypin (p = 0.81). Right, Cypin increases the number of excess secondary dendrites (ANODEV, p = 0.00002), and overexpression of PSD-95 blocks this effect. Error bars indicate SE.