Table 1. Degradation of variable-tail substrates in the bacterial cytoplasm.

Sequences of all substrate tails tested and the extent of degradation by ClpX^ΔNP in E. coli after 35 min. For substrates tested in multiple panels, the value presented is from the panel in which they first appear. Values are the average of three biological replicates ± S.D.

Substrate	Variable tail sequence	Fraction degraded in vivo
Gly12	GGGG GGGG GGGG	0.20 ± 0.05
GA	AGAG GGAG AGGA	0.88 ± 0.07
Titin	HLGL IEVE KPLY	0.78 ± 0.01
Basic	GKGR GKGR GKGR	0.83 ± 0.05
Acidic	GEGD GEGD GEGD	0.96 ± 0.01
LYV2-4	GLYV GGGG GGGG	0.82 ± 0.03
LYV4-6	GGGL YVGG GGGG	0.83 ± 0.01
LYV6-8	GGGG GLYV GGGG	0.65 ± 0.08
LYV8-10	GGGG GGGL YVGG	0.23 ± 0.01
LYV10-12	GGGG GGGG GLYV	0.2 ± 0.1
Tyr1	YGGG GGGG GGGG	0.3 ± 0.1
Tyr2	GYGG GGGG GGGG	0.49 ± 0.08
Tyr3	GGYG GGGG GGGG	0.80 ± 0.01
Tyr4	GGGY GGGG GGGG	0.80 ± 0.02
Tyr5	GGGG YGGG GGGG	0.8 ± 0.1
Tyr6	GGGG GYGG GGGG	0.4 ± 0.1
Tyr7	GGGG GGYG GGGG	0.32 ± 0.05
Tyr8	GGGG GGGY GGGG	0.20 ± 0.03
Ala4	GGGA GGGG GGGG	0.28 ± 0.03
Arg4	GGGR GGGG GGGG	0.39 ± 0.03
Asn4	GGGN GGGG GGGG	0.23 ± 0.01
Asp4	GGGD GGGG GGGG	0.20 ± 0.02
Cys4	GGGC GGGG GGGG	0.35 ± 0.02
Glu4	GGGE GGGG GGGG	0.27 ± 0.02
Gln4	GGGQ GGGG GGGG	0.41 ± 0.04
His4	GGGH GGGG GGGG	0.26 ± 0.01
Ile4	GGGI GGGG GGGG	0.78 ± 0.05
Leu4	GGGL GGGG GGGG	0.7 ± 0.1
Lys4	GGGK GGGG GGGG	0.36 ± 0.02
Met4	GGGM GGGG GGGG	0.6 ± 0.2
Phe4	GGGF GGGG GGGG	0.7 ± 0.1
Pro4	GGGP GGGG GGGG	0.19 ± 0.03
Ser4	GGGS GGGG GGGG	0.24 ± 0.04
Thr4	GGGT GGGG GGGG	0.24 ± 0.01
Trp4	GGGW GGGG GGGG	0.5 ± 0.1
Val4	GGGV GGGG GGGG	0.7 ± 0.1
Ala1	AGGG GGGG GGGG	0.41 ± 0.03
Ala1 + 4	AGGA GGGG GGGG	0.84 ± 0.03
Ala2 + 4	GAGA GGGG GGGG	0.88 ± 0.01
Ala3 + 4	GGAA GGGG GGGG	0.88 ± 0.01
Ala4 + 5	GGGA AGGG GGGG	0.87 ± 0.02
Ala4 + 6	GGGA GAGG GGGG	0.7 ± 0.1
Ala4 + 7	GGGA GGAG GGGG	0.51 ± 0.09
Ala4 + 8	GGGA GGGA GGGG	0.31 ± 0.04
Ala4 + 9	GGGA GGGG AGGG	0.24 ± 0.07
Ala4 + 10	GGGA GGGG GAGG	0.29 ± 0.07
Ala4 + 11	GGGA GGGG GGAG	0.33 ± 0.06
Ala4 + 12	GGGA GGGG GGGA	0.31 ± 0.04