Figure 1. Intracytoplasmic delivery of siRNA by peptide nanoparticles in pancreatic and colorectal cancer is spatially separate from lysosomes and highly efficient.

(A) Confocal microscopy demonstrates diffuse cell uptake of fluorescent tagged siRNA bearing NPs (pink) at 12 hours in CT26 cancer cells (cell wall cyan). (B) Confocal microscopy focusing on a single KPC-1 cancer cell (cell wall cyan) demonstrates accumulation of fluorescent signal (pink) in the cytoplasmic compartment, distinct from lysosomes (yellow), after administration of fluorescent siRNA-bearing peptide NPs. (C) Representative flow cytometry plot showing penetration of siRNA into the cytoplasm of KPC-1 pancreatic cancer.