Figure 16.

Hsp27 gene silencing upon delivery of different ssiRNAs (50 nM) mediated by G₅ TEA-core PAMAM (N/P = 10) to PC-3 cells (a), MDA-MB-231 cells (b), and MCF-7 cells (c). Checked bars: Data for ssiRNA with n = 5; solid bars: Data for ssiRNA with n = 7. In these experiments, vinculin was used as reference, and non-treated cells were used for control (green solid bar). Data for non-sticky siRNA (A₂/T₂) are also shown for comparison (red solid bar). MDA-MB-231 and MCF-7 are two different breast cancer cell lines. Redrawn from [57], with permission of the American Chemical Society.