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Flow cytometry of Leishmania amazonensis treated with 7.93 µg/mL of Endlicheria bracteolata essential oil to evaluate the mitochondrial membrane potential (ΔѰm). (A) Promastigotes captured in the gated region. (B–G) representative histograms of non-treated promastigotes incubated with TMRE showing ΔѰm intact in R2 and change of ΔѰm in R3. Promastigotes untreated (B) and treated for 2 h (C), 4 h (D), 8 h (E), 16 h (F), and 24 h (G).
