Figure 1. Myt1 family homolog ZTF-11 is expressed in neuronal precursors.

(A) Schematic showing ztf-11::gfp (wy1100). ZF, C2HC zinc-finger domain. ZF1, ZF1 zinc-finger domain. Magenta line underneath the second ZF denotes the area deleted in tm2315. See also Figure 1—figure supplement 1 for evolutionary conservation of Myt1 family proteins. (B) ZTF-11::GFP expression in neuronal precursor populations during embryogenesis. Also see Video 1. (C) Quantification of ZTF-11::GFP expressing cells in neuronal precursors and non-neuronal precursors at 350 cell stage. A neuronal precursor was defined by any cells giving rise to non-pharyngeal neurons. (D) Selected lineage diagrams showing correlation between ZTF-11::GFP expression and terminal cell fates in selected sub-lineages. Each dot under the line represents the ultimate cellular fate. In many cases, cells undergo additional round of cell division past automated lineage tracing and result in two daughter cells (indicated by two dots under each line). See also Supplementary file 1 for full lineage diagram with ZTF-11::GFP expression and their terminal fates.

Figure 1—figure supplement 1. Myt1 family proteins share conserved C2HC zinc-finger domains.

(A) Amino acid conservation of C2HC zinc-finger domains from representative Myt1 family proteins. Identical amino acid are in red and conserved amino acids are in yellow. Note the zinc ion stabilizing residues that define C2HC zinc-finger. Alignment was generated using T-Coffee and visualized by ESPript. (B) Constraint-based Multiple Alignment (COBALT) of representative Myt1 family proteins. Strongly conserved regions are in red, weakly conserved regions are in blue, and non-conserved regions are in gray.

Figure 1—figure supplement 2. ZTF-11 is expressed in postembryonic neuroectoblasts.

Representative confocal images of endogenous ZTF-11 expression during early larval development. Cells were identified based on lineage and position.