Figure 3. CD8α⁺ cDC1s Contain Less Listeria in Dock8^−/−Mice but Not Because of a DC-Intrinsic Defect.

(A) WT and Dock8^−/− mice were i.v. infected with 5–10 × 10⁸ Lm-GFP. 4 h later, the mice were sacrificed to measure intracellular Listeria in CD8α⁺ cDC1s. **p < 0.01. Results are representative of four independent experiments with n = 3 or 5/group.

(B) Bone-marrow-derived dendritic cells (BMDCs) generated from WT or Dock8^−/− mice were in vitro infected with Lm-GFP at MOI 5, and the BMDC Listeria load was analyzed based on GFP expression by flow cytometry (top dot plots). Freshly prepared splenocytes from naive WT or Dock8^−/− mice were in vitro infected with Lm-GFP at different MOIs, and the CD8α⁺ cDC1 bacterial load was gauged by GFP expression (bottom graph). Results are representative of three independent experiments.

(C) DC-Dock8^−/− (CD11c^cre-Dock8^fl/fl) and (Dock8^fl/fl) Cre control mice were i.v. infected with 5–10 × 10⁸ Lm-GFP. 4 h later, the mice were sacrificed to measure intracellular Listeria in CD8α⁺ cDC1s. Results are representative of two independent experiments with n = 3 or 5/group.

(D) DC-Dock8^−/− (CD11c^cre-Dock8^fl/fl) and Cre− control mice were i.v. infected with 10^s live rLm-OVA. The burdens of rLm-OVA in the spleen were determined by CFUs 3 days after infection, ns, not significant. Results are representative of three independent experiments with n = 4 or 5/group. Data are means ± SD. See also Figure S3.