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. 2019 Jul 29;8:e48788. doi: 10.7554/eLife.48788

Figure 5. Lmx1b temporally controls postnatal 5-HT terminal arborization.

(A) Schematic of tamoxifen inducible targeting of Lmx1b at postnatal day (P)3. (B) Sagittal view of cingulum shows fully formed long-range axon routes in P3 targeted Lmx1bicKO mice compared to iControls. Scale bars, 200 µm. (C) Coronal sections of hippocampus in P3 targeted Lmx1bicKO mice compared to iControls. Dashed boxed region: higher magnification image at right highlighting reduced TdTomato+ axons in Lmx1bicKO SLM. Scale bars, 200 µm (low magnification), 50 µm (high magnification). SLM, stratum lacunosum moleculare; DG, dentate gyrus. (D) Coronal sections of cortex of P3 targeted Lmx1bicKO mice compared to iControls. Imaris tracing; right panels. Scale bars, 100 µm. (E) Decreased TdTomato+ arbors detected in P3 targeted Lmx1bicKO PVT compared to iControls (arrows). Scale bars, 50 µm.

Figure 5.

Figure 5—figure supplement 1. P3 targeted Lmx1bicKO mice display normal 5-HT axon routing but decreased 5-HT terminal arbors.

Figure 5—figure supplement 1.

(A) Coronal view of SCS and cingulum demonstrating complete formation of long-range axon routes in P3 targeted Lmx1bicKO mice compared to iControls. Scale bars, 100 µm. (B) Quantification of axons within SCS and cingulum tracts, cortex, and hippocampus of postnatal targeted P3 iControls and P3 Lmx1bicKO mice (n = 3, iControl; n = 3, Lmx1bicKO mice). Unpaired t-test with Welch's correction; axon tracts, p=0.1474; cortex, p=0.04; hippocampus, p=0.0277. Data are represented as mean ± SEM.