Malaria continues to have a devastating impact on human health. With growing resistance to insecticides and antimalarial drugs, as well as climate change predictions indicating expansion of vector territories, the impact of malaria is likely to increase. Additional insights regarding pathogen migration through vector mosquitoes are needed to develop novel methods to prevent transmission to new hosts. Pathogens, including the microbes that cause malaria, must invade the salivary glands (SGs) for transmission. Since SG traversal is required for parasite transmission, SGs are ideal targets for transmission-blocking strategies. The work presented here highlights the role that mosquito SG architecture plays in limiting parasite traversal, revealing how the SG transmission bottleneck is imposed. Further, our data provide unprecedented detail about SG-sporozoite interactions and gland-to-gland variation not provided in previous studies.
KEYWORDS: malaria, mosquito, salivary gland, sporozoite
ABSTRACT
Plasmodium sporozoites (SPZs) must traverse the mosquito salivary glands (SGs) to reach a new vertebrate host and continue the malaria disease cycle. Although SGs can harbor thousands of sporozoites, only 10 to 100 are deposited into a host during probing. To determine how the SGs might function as a bottleneck in SPZ transmission, we have characterized Anopheles stephensi SGs infected with the rodent malaria parasite Plasmodium berghei using immunofluorescence confocal microscopy. Our analyses corroborate findings from previous electron microscopy studies and provide new insights into the invasion process. We identified sites of SPZ accumulation within SGs across a range of infection intensities. Although SPZs were most often seen in the distal lateral SG lobes, they were also observed in the medial and proximal lateral lobes. Most parasites were associated with either the basement membrane or secretory cavities. SPZs accumulated at physical barriers, including fused salivary ducts and extensions of the chitinous salivary duct wall into the distal lumen. SPZs were observed only rarely within salivary ducts. SPZs appeared to contact each other in many different quantities, not just in the previously described large bundles. Within parasite bundles, all of the SPZs were oriented in the same direction. We found that moderate levels of infection did not necessarily correlate with major SG disruptions or abundant SG cell death. Altogether, our findings suggest that SG architecture largely acts as a barrier to SPZ transmission.
INTRODUCTION
Malaria remains the vector-borne disease most harmful to human health, causing more than 400,000 annual deaths from hundreds of millions of annual infections (1). This impact comes despite gains made against the malaria death toll over the past 15 years, resulting primarily from insecticide spraying and bed net treatment campaigns. Unfortunately, these gains have diminished over the past 5 years with the rise of insecticide resistance (2). Research into new insecticides, potential malaria vaccines, and genetic strategies is ongoing and holds great promise (3–5).
The identification of mosquitoes as the vectors for Plasmodium (6) marked the beginning of research to understand their complex relationship. Much is now known about the journey of the malaria parasite through the mosquito (7–9). Female mosquitoes acquire Plasmodium during a blood meal from a previously infected host. Following gamete differentiation and fertilization, the ookinete traverses the midgut epithelium and forms an oocyst on the outer surface. Thousands of sporozoites (SPZs) develop within the oocyst and are released during oocyst rupture and carried by the hemolymph throughout the body cavity. Sporozoites selectively bind to and invade the salivary glands (SGs), where they accumulate in the secretory cavities, the shared lumen, and the salivary duct. SPZs can then be transmitted to a new host along with saliva during subsequent blood meals (8, 10).
The SGs are a key tissue within the vector (11), representing a membranous and cellular gateway that must be traversed for SPZs to gain access to the next host. Studies of mosquito SG biology to date have emphasized morphological descriptions by electron microscopy (EM) (12–16), identification of saliva components (17), or investigation of surface proteins and other molecules (18–20) that contribute to sporozoite invasion. Interestingly, SG cell death can limit disease transmission indirectly through an effect on parasite development in the midgut (21). Sporozoite ejection has been visualized by live imaging (22) and was found to occur even with sugar feeding (23).
Prior studies have noted the disparity between the very high numbers of SPZs found within mosquito SGs and the much smaller number ejected into the mammalian host (22, 24, 25). Numbers of SG SPZs range widely among field populations (26). Our previous studies (surveying gland-to-gland variations in SG architecture, determining how the adult SG forms the secretory portion of the salivary duct, and describing how SG secretory cells acquire their unusual cup-shaped morphology [27, 28]) suggested that cell architecture variation may play a role in the journey of sporozoites through the SGs. Prior electron microscopy analysis included limited sample sizes and did not allow visualization of multiple molecular markers. Therefore, we applied immunofluorescence (IF) confocal microscopy to hundreds of infected mosquito SGs to study SPZ localization and SG features at multiple infection intensities. We found that several SG architectural features create barriers to SPZ traversal.
RESULTS
Sporozoite localization within infected salivary glands.
We dissected, stained, and imaged Anopheles stephensi salivary glands (SGs) between 14 and 29 days postinfection with one of several strains of Plasmodium berghei (see Materials and Methods) to determine sporozoite (SPZ) localization (Fig. 1). Distal lateral (DL) lobes were the primary site of SPZ occupancy (Fig. 1A, panels i, ii, and iv). SPZs were observed in association with the basement membrane (Fig. 1A, panels ii and iv to vi), pockets of saliva between the basement membrane and secretory cells (Fig. 1A, panel iv), inside secretory cell cytoplasms (Fig. 1A, panels iii and iv), in secretory cavities (Fig. 1A, panels ii and iv), next to the salivary duct (Fig. 1A, panel iv), inside the SG lumen (Fig. 1A, panel iii), and in the salivary duct (Fig. 1A, panel iv [inset]). Notably, SPZs were frequently seen in proximal lateral (PL) lobes (Fig. 1B, panel i, asterisk) and medial (M) lobes (Fig. 1B, panel ii, asterisk). In 783 SG lobes (365 DL, 149 M, and 269 PL) imaged (Fig. 1D), SPZs were observed in about 73% of DL lobes, 47% of PL lobes, and 44% of M lobes from infected SGs (Fig. 1C). Among the imaged SGs, 52 DL, 23 M, and 39 PL lobes were from SGs that completely lacked SPZs (Fig. 1D). Numbers of SPZs differed between SGs and lobes of the same mosquito (Fig. 1E). In one DL lobe, a single SPZ was seen near a fused shut salivary duct (Fig. 1E, panels ii and iii); in the other DL lobe of the same mosquito, about 15 SPZs were present (Fig. 1E, panel iv). In another DL lobe, secretory cell cytoplasms (Fig. 1F, slice), but not secretory cavities (Fig. 1F slice, asterisks), were packed full of SPZs. Counts in this lobe revealed that approximately 40 SPZs can fit within a single DL lobe secretory cell cytoplasm (Fig. 1F). SPZs were rarely observed within the salivary duct and only in small quantities (Fig. 1G).
To better address SG localizations or structural features associated with a variety of SPZ numbers, we scored each lobe independently and assigned SG lobes into bins based on the number of SPZs present (Fig. 2A and C) as follows: Zero, 0 SPZs; Lo (low numbers of SPZs), 1 to 10 SPZs; Me (medium), 11 to 100 SPZs; Hi (high), 101 to 1,000 SPZs; Vh (very high), >1,000 SPZs. Lobes with lower numbers of SPZs were more prevalent than those with higher numbers (Fig. 2B). In lobes with low quantities, SPZs were most frequently observed in association with the basement membrane; in lobes with higher quantities, SPZs were most frequently seen in the secretory cavities (Fig. 2C). We counted several types of structural and morphological defects. Saliva accumulations between secretory cells and the basement membrane (Fig. 2D), basement membrane disruptions (Fig. 2E), and secretory cell cytoplasmic disruptions (Fig. 2F) were each observed most frequently in the DL lobes.
The low frequencies and numbers of SPZs seen in the salivary duct were unexpected, so we asked if providing a second blood meal to previously infected mosquitoes at a time of high SPZ occupancy (day 23 postinfection) of SGs would alter SPZ localization (Fig. 2G). Surprisingly, we did not observe any differences in SPZ localization. SPZs were seen at the basement membrane, in secretory cell cytoplasms, and in secretory cavities (Fig. 2G, arrows). A small increase in the levels of Me and Hi infection was seen after a second blood meal (Fig. 2I), consistent with their 2-h wait compared to paired “no second blood meal” mosquitoes. Similar rates of SG structural defects and salivary duct SPZ occupancy were observed regardless of whether or not a second blood meal was provided (Fig. 2J). Importantly, no increase in salivary duct occupancy by SPZs was seen after the second blood meal.
We also considered trends in the relationships between time postinfection, SPZ organization (individuals versus bundles), infection intensity, and structural defects in our data (see Fig. S1A to E in the supplemental material). Infection intensity peaked at days 23 to 24 postinfection (Fig. S1A), in line with Johns Hopkins Bloomberg School of Public Health Malaria Research Institute (JHMRI) Insectary condition estimates (29). Individualized SPZs were more prevalent than bundled SPZs, and the numbers of both peaked with age (Fig. S1B) and high infection intensity (Fig. S1C). Higher infection intensity was associated with more occurrences of basement membrane disruptions (Fig. S1D) and secretory cell cytoplasmic disruptions (Fig. S1E) in all lobe types.
Finally, we interrogated the effects of processing and imaging SG tissue samples for immunofluorescence on SG structure and SPZ localization using differential interference contrast (DIC) imaging (Fig. 2H). SPZs were visible as individuals (Fig. 2H, arrowheads) or in groups (Fig. 2H, arrows), with the highest numbers of SPZs seen in the secretory cavities. No SPZs were observed in the salivary ducts.
CSP localization during salivary gland invasion and egress.
To better understand SPZ ingression and egression events in SGs, we stained infected SGs with an antibody that recognizes both processed and nonprocessed forms of circumsporozoite protein (CSP). CSP is the major protein present on the SPZ coat, is involved in motility, and may promote SG invasion (16, 30–33). We observed three CSP staining patterns: SPZs with a thick CSP coat (Fig. 3A, panel iv, arrow), SPZs with a thin CSP coat (Fig. 3A, panel v, arrow), and tracks likely composed of shed CSP within cells (Fig. 3A, panel iv, arrowhead). These data support prior indications that CSP coat morphology correlates with SG invasion status (31). A thick CSP coat is present on SPZs that have not invaded a SG cell, whereas a thin CSP coat indicates SPZs that have invaded a SG cell. Infrequently, instances of thick CSP-coated SPZs were observed in secretory cell cytoplasms (Fig. 3A, panels ix and x). Strong accumulations of CSP signal were observed in lobes containing basement membrane and cellular disruptions (Fig. 3B, panel vii). Sometimes, both SPZ coat-associated CSP (arrows) and shed CSP (arrowheads) were present (Fig. 3B, panel viii). Secretory cell cytoplasmic markers were regularly found in association with the coat of some SPZs after invasion (Fig. 3B, panel v, and C). These results suggest that the CSP-containing coat is largely shed as SPZs invade secretory cells but that it may be retained when SPZs enter through a large disruption. Accordingly, CSP staining (16, 30, 31) was greatly reduced internally to the basement membrane along a single SPZ during invasion (Fig. 3D; see also Movie S1). Within SG secretory cells, we observed two sites of shed CSP accumulation (Fig. S2): at the apical surface (Fig. 3D, panel viii; see also Fig. S2C to H, yellow arrows) and in a more basal, cytoplasmic compartment showing relative depletion of cellular markers (Fig. S2D to I, white arrows) (mitochondrial transcription factor A [mtTFA], purple; wheat germ agglutinin [WGA], blue). These results suggest that CSP shedding continues during SG secretory cell invasion, traversal, and egress.
Sporozoites accumulate at physical barriers.
SPZs accumulated at sites of physical barriers within the SGs (Fig. 4A). The most notable obstruction was the salivary duct wall within proximal secretory cells of the DL lobe. SPZs were frequently observed oriented perpendicularly to the thick chitinous secretory duct wall, which extends about halfway into DL lobes, just interior to the secretory cavities. A periductal space is visible between the apical edge of secretory cells and the salivary duct when sufficient saliva is present. Near the basal surface of this DL lobe (Fig. 4A, panels ii and iii), SPZs were mainly individualized and randomly oriented. Internally, this lobe showed a defect in the usually open interface between most secretory cavities and the lumen (Fig. 4A, panels iv and vi). In this example, the chitinous duct appeared to extend around the outer lumenal interface, dividing the lumenal and secretory cavity territories in all but one DL lobe cell (Fig. 4A, panel iv, arrow). SPZs were lined up perpendicularly to this extended chitinous boundary (Fig. 4A, panels v and ix). One SPZ was seen crossing the boundary through a small disruption (Fig. 4A, panels v to vii, arrows), and a SPZ was observed nearby inside the salivary duct (Fig. 4A, panel ix). We found additional examples of SG cell architectures acting as barriers to SPZ traversal. One contained two barrier architectures (Fig. 4B). In one DL lobe, the salivary duct terminus was fused shut (Fig. 4B, panel ii, arrow). SPZs filled the secretory cavities (Fig. 4B, panels ii and iii), but no SPZs were observed inside the duct. In another DL lobe, a widened, thickened salivary duct was seen to connect to a small, round, mispositioned lumen (Fig. 4B, panel iv [inset 1, Lu]). SPZs were seen in secretory cavities aligned perpendicularly to the duct (Fig. 4B, panel iv). SPZs were present in the lumen (Fig. 4B, panel iv, asterisk), which might have been made accessible by a secretory cell disruption enriched for SPZ green fluorescent protein (GFP) (Fig. 4B, panel iv, arrow; see also panel iv, inset 2), forming a lumenal-periductal space passage. One DL lobe (Fig. 4C) contained a circular disruption in the middle of the lumen at the salivary duct terminus (Fig. 4C, panels i and vi) which blocked SPZ traversal from secretory cells to the lumen and/or from the lumen to the duct (Fig. 4C, panels ii to iv). WGA signal, likely from secreted O-GlcNAcylated proteins, was enriched proximally to the disruption within the SG (Fig. 4C, panel v). SPZs did not traverse the salivary duct wall in the proximal region (Fig. 4C, panel vi). Finally, one DL lobe (Fig. 4D) was corkscrewed and contained a fused salivary duct near the basal tip of the lumen (Fig. 4D, panel ii, arrow). SPZs were clustered around, but not found inside, the fused duct. These data indicate that SG architectural features can hinder SPZ invasion and egress.
Salivary gland morphology, cell damage, and secretion consequences from infection.
To look for effects of SPZ invasion on SG morphometry, we measured SG lobe maximal width and maximal length under various conditions. In general, lengths and widths were similar across days postinfection and levels of infection intensity for all three lobe types (Fig. S1F and G). Small differences were seen in DL lobe length, DL lobe width, and PL lobe width in comparisons of SGs processed for immunofluorescence to directly mounted samples (Fig. S1H). To explore this further, we created scatter plots mapping each lobe’s length and width during processing for immunofluorescence (Fig. S1I) or direct mounting (Fig. S1J). Strikingly, the directly mounted samples had much more consistent lengths and widths for all three lobes than the samples processed for IF (Fig. S1I and J). This suggests that the major effect of our 90-s acetone permeabilization for antibody staining was a weakening of the SG basement membrane that allowed SGs to splay more randomly when the coverslip was placed during mounting. No evidence of tissue shrinking during sample processing for staining was observed. Thus, we found that SG invasion had little impact on overall SG morphology.
We assessed the extent of SG morphological alteration with SPZ invasion. We found examples of robustly invaded SGs with nearly unperturbed structures and very little evidence of cell death, as well as examples of SGs with many disrupted cells and decreased saliva protein abundance (Fig. 5). For example, one DL lobe (Fig. 5A) contained many secretory cavity SPZs (Fig. 5A, panels vi and xi) but had only three small basement membrane disruptions (Fig. 5A, panels iv, v, and viii) and two cells positive for the apoptosis marker cleaved caspase 3 (CC3, purple; Fig. 5A, panels ix and x). These data indicate that extensive SG invasion by SPZs in the absence of large numbers of dying cells can occur, as previously suggested (16). Another sample DL lobe (Fig. 5B, panel i) contained many SPZs in the proximal portion but many fewer in the distal portion (Fig. 5B, panel ii). In the proximal DL lobe, secretory cell cytoplasms were disrupted (Fig. 5B, panel v, arrows), saliva accumulated basally, and SPZs were observed in the basal saliva (Fig. 5B, panel v), whereas the typical cup shape of secretory cells in close association with the basement membrane was preserved in the distal DL lobe (Fig. 5B, panel vii). Staining for Anopheles antiplatelet protein (AAPP, purple) was much weaker in the disrupted proximal DL lobe (Fig. 5B, panel vi) than in the intact distal DL lobe (Fig. 5B, panel viii). These data suggest that some invaded SGs sustain extensive cellular damage that reduces secretory protein abundance.
Sporozoite interactions inside salivary glands.
We observed several types of SPZ interactions within SGs beyond bundling (Fig. 6A). SPZs were found in contact in a variety of quantities, from 2 SPZs (Fig. 6A, panel vi) to 6 to 8 (Fig. 6A, panel vii) or 10 to 12 (Fig. 6A, panel v). In several SGs, we observed groups of SPZs with circling/spiraling organization within secretory cavities (Fig. 6B, panel iv, yellow arrow, and panels v to vi; see also Movies S2 and S3 in the supplemental material). The role of circling/spiraling in SPZ biology is currently unknown. Often, both individualized SPZs (Fig. 6C, panels ii and iii) and bundled SPZs (Fig. 6C, panels iv to vii) were found within the same DL lobe. Individualized SPZs were observed more frequently and in greater numbers than bundled SPZs (Fig. 6D). Whereas individualized SPZs were oriented randomly, as revealed by the SPZ GFP signal (Fig. 6C, panel iii), staining for the Plasmodium thrombospondin repeat-associated protein (TRAP), a motility and invasion protein (34, 35), demonstrated that all SPZs within a bundle were oriented in the same direction (Fig. 6C, panel vii). We also observed an example of multiple, differentially oriented bundles within a single secretory cavity (Fig. 6E, panel iii, arrows). A bundle of SPZs, elsewhere in this lobe, enriched at one end for CSP signal (Fig. 6E, panel iv, arrow) was observed within a basement membrane disruption. These observations raise the possibility that bundling may allow SPZs to pool their enzymatic and/or mechanical efforts to move through physical barriers.
DISCUSSION
In this report, we describe the many barriers to SG traversal by Plasmodium berghei SPZs presented by Anopheles stephensi SG architecture (Fig. 7). Typically, SPZs associated with the basement membrane (Fig. 7, step 1) invade SG secretory cells (Fig. 7, step 2), exit secretory cells and enter secretory cavities (Fig. 7, step 3), move from secretory cavities into the larger central lumen (Fig. 7, step 4), and enter the salivary duct through the open terminus (Fig. 7, step 5). We identified many variant architectural features with SGs that impeded sporozoite invasion, including fused salivary duct termini (Fig. 7, step 6), perilumenal chitin enrichments (Fig. 7, step 14, red asterisk), and bundled SPZs partially blocking the secretory cavity exit (Fig. 7, step 16). Accumulations of sporozoites were seen associated with these and other features, including secretory cell cytoplasms (Fig. 7, sections 9 and 10), aberrant basal compartments of saliva occurring between secretory cells and the basement membrane (Fig. 7, step 13), and circling arrangements within select secretory cavities (Fig. 7, step 18). Unnatural routes of entry or large secretory cell and/or basement membrane disruptions (Fig. 7, sections 7 and 8) might allow SPZs direct access to secretory cell cytoplasms, secretory cavities, or the lumen without secretory cell invasion in certain SGs. Abnormal sporozoite traversal events were seen in individuals across cell cytoplasms (Fig. 7, step 12) and as bundles moving through aberrant chitin accumulations (Fig. 7, step 15) or the basement membrane to exit the SG (Fig. 7, step 17). Rounded (possibly constrained/misshapen during invasion [36] or dead or dysregulated [37] or lysed [38]) SPZs (Fig. 7, step 11) were observed both in association with the basement membrane and inside some secretory cells. Thus, SG cell architecture plays a critical role in determining SPZ availability for transmission.
Comparisons to prior studies.
Previous studies have used electron microscopy to analyze the localization of Plasmodium SPZs within infected SGs (12–15) as well as bundling (12). Posthuma and others observed Plasmodium falciparum SPZs invading (and inside) Anopheles stephensi SGs by electron microscopy (16, 39). Their results and ours largely agree. Their data suggest that SPZs pierce the basement membrane during invasion, as they showed no signs of membrane stretching or parasitophorous vacuole formation. Our data support this interpretation of the mechanism of entry. We did not observe membrane stretching. However, we did see that a halo of secretory cell cytoplasmic material was retained on the SPZ coat for some time after invasion (Fig. 3B, panel v, and C, panels iii and iv). Further, they observed CSP accumulation inside SG cells, such as we also observed (Fig. 3; see also Fig. S2 in the supplemental material). Thus, the different imaging techniques (immuno-EM and immunofluorescence) gave consistent results.
Pimenta and others provided the most complete description to date of the steps in SG invasion in Aedes aegypti infected with Plasmodium gallinaceum. Their model is still widely accepted and applied to many mosquito/Plasmodium combinations (12). In contrast to our data, they suggested that SPZs interact with the basement membrane and enter the SG through formation of a junction. During entry, the sporozoite acquires a vacuole of SG cellular material that is subsequently lost. Upon secretory cell exit, the sporozoite evaginates the apical membrane and enters the secretory cavity encased in a vacuole that is subsequently lost. Sporozoites then accumulate and bundle inside secretory cavities. Groups of bundled parasites then enter the salivary duct through an unknown mechanism that may involve a chitinase (12). Differences between our results and theirs likely reflect vector/parasite species differences.
Our results revealed both similarities and differences between Aedes SGs and Anopheles SGs with respect to Plasmodium invasion. Rather than a membranous vacuole forming around SPZs as they enter the SG cells using a moving junction (12), our findings and those reported previously others (16) suggest that SPZs puncture SG membranes and acquire a halo of cytoplasmic material that associates with the sticky SPZ coat following invasion (Fig. 3B, panel v, and C, panels iii to v) until it is lost by coat shedding. Both studies also determined that SPZs bundle inside secretory cavities, and we were able to capture both new small-scale SPZ interactions (Fig. 6A) and a new SPZ organization pattern, a circling/swirling pattern that could be a precursor to the bundles that were observed in both systems (Fig. 6B, panel vi; see also Movies S2 and S3 in the supplemental material). Aedes female SG duct termini are fused shut, making it unclear how sporozoites enter the salivary duct (12). We found fused duct termini in 3% of Anopheles SGs (24, 25) (this study) and very little evidence of how SPZs entered a closed duct. Whereas Pimenta and others suggested previously that hundreds of SPZs could occupy an Aedes SG secretory cell, we found that only about 40 SPZs could fill a typical Anopheles secretory cell cytoplasm (Fig. 1F). Further, we identified SPZ accumulations associated with many architecture features acting as barriers to SG traversal (Fig. 7).
Connections across stages of the malaria cycle.
SPZ invasion was not always associated with large numbers of dying (cleaved caspase 3-positive) cells or basement membrane disruptions, even with large numbers (Fig. 5A). SPZs may reuse the same puncture sites for SG entry, without further SG damage, unless there are overwhelming numbers of sporozoites (Fig. 5B). Alternatively, SPZs may suppress apoptotic signals during initial invasion events, as happens during hepatocyte invasion (40). It may also be possible that the SGs can restore damaged basement membranes through deposition of new materials (12, 15, 16).
We identified sites in mosquito SG cells where the sporozoite motility and invasion protein CSP accumulates after it has been shed by the parasite during traversal. These sites (see Fig. S3 in the supplemental material), at the apical surface and a subcellular compartment relatively depleted of mitochondria and O-GlcNAcylated proteins, suggest possible interactions between CSP and the SG secretory cell. Singh and others found that shed CSP in hepatocytes blocked the production of NF-κB, dampening the immune response elicited by invaded cells (41). Further study will be required to determine if shed sporozoite CSP acts to dampen innate immunity in mosquito SGs. Interestingly, TRAP, like CSP, is also shed during motility and infection (35); however, we detected only one SPZ TRAP staining pattern (Fig. 6C).
The extent to which SPZs communicate within mosquito SGs is unclear. Certain SPZ behaviors observed (bundling, bundle orientation, circling, traversing cellular barriers) suggest possible molecular communication. Other SPZ observations (consistent orientation toward the lumen, clustering at closed salivary duct termini) suggest that saliva flow and a possible molecular signal drive SPZs to leave the SGs.
Membrane barriers and SPZ maturation in the SG.
Another key finding in our study is the existence of disruptions of large basement membranes, and sometimes secretory cells, in some infected SGs capable of allowing SPZs to bypass either or both of the required traversals of membranes (the basement membrane and the apical secretory cell membrane). Smaller disruptions had been observed previously (15, 16). This could facilitate mixed populations of SPZs within some SGs possessing mixed infection capability (thickly and thinly CSP-coated SPZs; Fig. 3A). SPZs may undergo a maturation process in the SGs to become capable of hepatocyte invasion in the mammalian host (42–44); SG membrane traversal may play a role in this process.
Model for SG architecture as a roadblock to SPZ transmission.
The disparity between SG sporozoite numbers (reaching well into the thousands) and numbers of SPZs deposited into mammalian skin (10 to 100) has been well documented (22, 24). Work in other laboratories has suggested a high degree of transmission variability between individual mosquitoes (24). Further, pioneering live-imaging studies have also suggested that the first blood meal taken by mosquitoes capable of transmission is less successful than subsequent meals and that the SPZs that are transmitted already reside in the mouthparts just prior to probing (22). Our data suggest that SG architectural features limit the numbers of SPZs available for transmission.
Our current data suggest that SG invasion and traversal represent upstream, rate-limiting steps for parasite transmission. Once the route to the salivary duct is open, the pump muscle may force saliva (along with free SPZs) quickly through to the mouthparts, where SPZs then remain until the next probing event. Saliva flow is likely a major contributor to SPZ movement within SGs (22), but a diffusible chemical signal may also contribute (45). This signal could elicit the consistent orientation of SPZs toward the salivary duct that we observe in lumens bordered by aberrant chitin-positive regions (Fig. 1A, panel iv, and E, panel iii; see also Fig. 4A, panels iv to ix, and B, panels ii and iv).
MATERIALS AND METHODS
Mosquito husbandry.
Anopheles stephensi (Dutch strain) mosquitoes were maintained on 10% sucrose (available ad libitum) at 28°C and 75% humidity in a walk-in environmental chamber in the Johns Hopkins Bloomberg School of Public Health Malaria Research Institute Insectary using standard procedures (46). Sucrose was administered in a small glass bottle with a cotton wick prior to the first blood meal.
Blood meals and infection.
Infective blood meals were conducted by JHMRI Insectary staff, primarily Godfree Mlambo, as previously described (47). After an infective blood meal, 10% sucrose was made continuously available to mosquitoes in a soaked cotton pad. In one experiment, a second, noninfective blood meal was similarly given to mosquitoes infected with Plasmodium berghei 23 days prior. The parasite strains used included the following: (i) wild-type (WT) P. berghei (ANKA strain; Fig. 1A, B, and E, Fig. 3, Fig. 4C and D, and Fig. 6A and E; see also Fig. S2 and Data Set S1 in the supplemental material) (48); (ii) P. berghei (ANKA strain) expressing GFP under the control of the elongation factor 1 alpha (eef1aa) promoter (Fig. 1; see also Fig. 2 and Fig. S1 [49]); (iii) P. berghei (ANKA strain) expressing GFP and luciferase under the control of the eef1aa promoter (Fig. 1F; see also Fig. 2A, G, and H, Fig. 4A and B, Fig. 5, and Fig. 6B and C and Fig. S3 [49]); and (iv) P. berghei (ANKA) expressing GFP under the control of the P. berghei HSP70 promoter (Fig. 1; see also Fig. 2 and Fig. S1 and Data Set S1 [50]). Similar results were observed across all strains tested.
Dissections, staining, and mounting.
Salivary gland (SG) dissections, acetone permeabilization, tissue staining, and mounting were performed as described previously (27, 28). All primary antibodies were diluted 1:50, and all secondary antibodies were diluted 1:200. Secondary antibody staining in the absence of primary antibody staining showed very little background signal (see Fig. S3 in the supplemental material). Formaldehyde and glacial acetic acid fixation (28) was also tested (Data Set S1) to validate the results obtained by acetone permeabilization. Comparable results were observed. For the direct mounting experiment (Fig. 2; see also Fig. S2), dissected SGs, still attached to the head, were immediately added to a drop of glycerol on a microscope slide. Once 10 SGs were obtained, the tissues were positioned on the slide in glycerol and a cover slip was added.
Dyes and antibodies.
The dyes and primary antisera employed in this study were as follows: DAPI (4′,6-diamidino-2-phenylindole; Life Technologies) (1:50), Rh-WGA (Vector Labs) (1:40), Nile red (Sigma) (1:50), phalloidin-Alexa 488 (Life Technologies) (1:10), chicken anti-GFP (Abcam ab13990), mouse anti-CSP (gift from Photini Sinnis), rabbit anti-mtTFA (Santa Cruz H-203), rabbit anti-TRAP (gift from Photini Sinnis), rabbit anti-CC3 (catalog no. 9661; Cell Signaling), AAPP (gift from Hiroyuki Matsuoka), and rabbit anti-GM130 (Abcam ab30637). Secondary antibodies (goat anti-rabbit 488 [A11008] and goat anti-mouse 647 [A21235]) were from Life Technologies.
Confocal and bright-field microscopy.
Confocal imaging was conducted using a Zeiss LSM700 laser scanning confocal microscope housed in the Johns Hopkins University School of Medicine Microscope Facility. The step size used in three-dimensional (3D) image stack captures was 1 μm. Each figure is composed of representative images from between 6 and 20 imaged glands, selected from 30 to 120 dissected and stained glands per experiment, fixed, and immunostained between one and four different days. DIC microscopy of the directly mounted samples was conducted on a Zeiss Axiophot light microscope equipped with ProgRes C14PLUS image capture system beginning within 2 h after the start of dissections.
Image analysis and statistical analysis.
Image processing was completed in Zeiss Zen 2010, Adobe Photoshop CS4, and ImageJ (line scan). Quantification of sporozoite numbers and SG architecture features was done in Microsoft Excel. Statistical analyses and graphing were completed using Minitab 17 and Microsoft Excel. The original image stack capture files are available upon request. Raw data and analyses of infected SG quantification are provided in Data Set S1.
ACKNOWLEDGMENTS
We thank the members of the Andrew laboratory and Marcelo Jacobs-Lorena, Carolyn Machamer, and Katherine Wilson for discussions useful for the completion of this work. We thank Photini Sinnis (anti-CSP and anti-TRAP) and Hiroyuki Matsuoka (anti-AAPP) for generous gifts of antisera. We thank Marcelo Jacobs-Lorena, Chris Kizito, and Godfree Mlambo for assistance with mosquito rearing and infection.
Research reported in this publication was supported by the Office of the Director (OD) of the National Institutes of Health under award number S10OD016374 (to S. C. Kuo). Salary support and funding for reagents were provided by NIH R01 DE013899 (to D.J.A.). Salary support was also provided by a 2017–2019 Johns Hopkins Malaria Research Institute Postdoctoral Fellowship (to M.B.W.). We acknowledge the support of the Johns Hopkins Malaria Research Institute and Bloomberg Philanthropies.
Footnotes
Citation Wells MB, Andrew DJ. 2019. Anopheles salivary gland architecture shapes Plasmodium sporozoite availability for transmission. mBio 10:e01238-19. https://doi.org/10.1128/mBio.01238-19.
REFERENCES
- 1.World Health Organization. 2017. World malaria report 2017. World Health Organization, Geneva, Switzerland. [Google Scholar]
- 2.Cibulskis RE, Alonso P, Aponte J, Aregawi M, Barrette A, Bergeron L, Fergus CA, Knox T, Lynch M, Patouillard E, Schwarte S, Stewart S, Williams R. 2016. Malaria: global progress 2000–2015 and future challenges. Infect Dis Poverty 5:61–61. doi: 10.1186/s40249-016-0151-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 3.Hemingway J, Shretta R, Wells TNC, Bell D, Djimdé AA, Achee N, Qi G. 2016. Tools and strategies for malaria control and elimination: what do we need to achieve a grand convergence in malaria? PLoS Biol 14:e1002380. doi: 10.1371/journal.pbio.1002380. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 4.Wang S, Dos-Santos ALA, Huang W, Liu KC, Oshaghi MA, Wei G, Agre P, Jacobs-Lorena M. 2017. Driving mosquito refractoriness to Plasmodium falciparum with engineered symbiotic bacteria. Science 357:1399. doi: 10.1126/science.aan5478. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 5.Pike A, Dong Y, Dizaji NB, Gacita A, Mongodin EF, Dimopoulos G. 2017. Changes in the microbiota cause genetically modified Anopheles to spread in a population. Science 357:1396. doi: 10.1126/science.aak9691. [DOI] [PubMed] [Google Scholar]
- 6.Cox FE. 2010. History of the discovery of the malaria parasites and their vectors. Parasit Vectors 3:5. doi: 10.1186/1756-3305-3-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7.Phillips MA, Burrows JN, Manyando C, van Huijsduijnen RH, Van Voorhis WC, Wells T. 2017. Malaria. Nat Rev Dis Primers 3:17050. doi: 10.1038/nrdp.2017.50. [DOI] [PubMed] [Google Scholar]
- 8.Ghosh A, Edwards MJ, Jacobs-Lorena M. 2000. The journey of the malaria parasite in the mosquito: hopes for the new century. Parasitol Today 16:196–201. doi: 10.1016/S0169-4758(99)01626-9. [DOI] [PubMed] [Google Scholar]
- 9.Simonetti AB. 1996. The biology of malarial parasite in the mosquito: a review. Mem Inst Oswaldo Cruz 91:519–541. doi: 10.1590/s0074-02761996000500001. [DOI] [PubMed] [Google Scholar]
- 10.Hillyer JF, Barreau C, Vernick KD. 2007. Efficiency of salivary gland invasion by malaria sporozoites is controlled by rapid sporozoite destruction in the mosquito haemocoel. Int J Parasitol 37:673–681. doi: 10.1016/j.ijpara.2006.12.007. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 11.Mueller AK, Kohlhepp F, Hammerschmidt C, Michel K. 2010. Invasion of mosquito salivary glands by malaria parasites: prerequisites and defense strategies. Int J Parasitol 40:1229–1235. doi: 10.1016/j.ijpara.2010.05.005. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 12.Pimenta PF, Touray M, Miller L. 1994. The journey of malaria sporozoites in the mosquito salivary gland. J Eukaryot Microbiol 41:608–624. doi: 10.1111/j.1550-7408.1994.tb01523.x. [DOI] [PubMed] [Google Scholar]
- 13.Ando K, Kuraishii K, Nishikubo K, Asami T, Waidhet-Kouadio P, Matsuoka H, Chinzei Y. 1999. Sporozoite invasion of Plasmodium berghei rodent malaria parasite to the salivary glands of the vector mosquito, Anopheles stephensi: an electron microscopic study. Jap J Trop Med Hyg 27:7–12. doi: 10.2149/tmh1973.27.7. [DOI] [Google Scholar]
- 14.Sterling CR, Aikawa M, Vanderberg JP. 1973. The passage of Plasmodium berghei sporozoites through the salivary glands of Anopheles stephensi: an electron microscope study. J Parasitol 59:593–605. doi: 10.2307/3278847. [DOI] [PubMed] [Google Scholar]
- 15.Meis J, Wismans PGP, Jap PHK, Lensen AHW, Ponnudurai T. 1992. A scanning electron microscopic study of the sporogonic development of Plasmodium falciparum in Anopheles stephensi. Acta Tropica 50:227–236. doi: 10.1016/0001-706X(92)90079-D. [DOI] [PubMed] [Google Scholar]
- 16.Posthuma G, Meis JF, Verhave JP, Gigengack S, Hollingdale MR, Ponnudurai T, Geuze HJ. 1989. Immunogold determination of Plasmodium falciparum circumsporozoite protein in Anopheles stephensi salivary gland cells. Eur J Cell Biol 49:66–72. [PubMed] [Google Scholar]
- 17.Arcà B, Lombardo F, Struchiner CJ, Ribeiro J. 2017. Anopheline salivary protein genes and gene families: an evolutionary overview after the whole genome sequence of sixteen Anopheles species. BMC Genomics 18:153. doi: 10.1186/s12864-017-3579-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 18.Kappe SHI, Kaiser K, Matuschewski K. 2003. The Plasmodium sporozoite journey: a rite of passage. Trends Parasitol 19:135–143. doi: 10.1016/S1471-4922(03)00007-2. [DOI] [PubMed] [Google Scholar]
- 19.Kappe SH, Buscaglia CA, Nussenzweig V. 2004. Plasmodium sporozoite molecular cell biology. Annu Rev Cell Dev Biol 20:29–59. doi: 10.1146/annurev.cellbio.20.011603.150935. [DOI] [PubMed] [Google Scholar]
- 20.Perrone JB, DeMaio J, Spielman A. 1986. Regions of mosquito salivary glands distinguished by surface lectin-binding characteristics. Insect Biochem 16:313–318. doi: 10.1016/0020-1790(86)90041-7. [DOI] [Google Scholar]
- 21.Yamamoto DS, Sumitani M, Kasashima K, Sezutsu H, Matsuoka H. 2016. Inhibition of malaria infection in transgenic anopheline mosquitoes lacking salivary gland cells. PLoS Pathog 12:e1005872. doi: 10.1371/journal.ppat.1005872. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 22.Frischknecht F, Baldacci P, Martin B, Zimmer C, Thiberge S, Olivo-Marin J-C, Shorte SL, Ménard R. 2004. Imaging movement of malaria parasites during transmission by Anopheles mosquitoes. Cell Microbiol 6:687–694. doi: 10.1111/j.1462-5822.2004.00395.x. [DOI] [PubMed] [Google Scholar]
- 23.Brugman VA, Kristan M, Gibbins MP, Angrisano F, Sala KA, Dessens JT, Blagborough AM, Walker T. 2018. Detection of malaria sporozoites expelled during mosquito sugar feeding. Sci Rep 8:7545. doi: 10.1038/s41598-018-26010-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 24.Medica DL, Sinnis P. 2005. Quantitative dynamics of Plasmodium yoelii sporozoite transmission by infected anopheline mosquitoes. Infect Immun 73:4363–4369. doi: 10.1128/IAI.73.7.4363-4369.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 25.Churcher TS, Sinden RE, Edwards NJ, Poulton ID, Rampling TW, Brock PM, Griffin JT, Upton LM, Zakutansky SE, Sala KA, Angrisano F, Hill AVS, Blagborough AM. 2017. Probability of transmission of malaria from mosquito to human is regulated by mosquito parasite density in naïve and vaccinated hosts. PLoS Pathog 13:e1006108. doi: 10.1371/journal.ppat.1006108. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 26.Beier JC, Onyango FK, Ramadhan M, Koros JK, Asiago CM, Wirtz RA, Koech DK, Roberts CR. 1991. Quantitation of malaria sporozoites in the salivary glands of wild Afrotropical Anopheles. Med Vet Entomol 5:63–70. doi: 10.1111/j.1365-2915.1991.tb00522.x. [DOI] [PubMed] [Google Scholar]
- 27.Wells MB, Villamor J, Andrew DJ. 2017. Salivary gland maturation and duct formation in the African malaria mosquito Anopheles gambiae. Sci Rep 7:601. doi: 10.1038/s41598-017-00672-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 28.Wells M, Andrew D. 2015. Salivary gland cellular architecture in the Asian malaria vector mosquito Anopheles stephensi. Parasit Vectors 8:617. doi: 10.1186/s13071-015-1229-z. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 29.Sinden RE, Dawes EJ, Alavi Y, Waldock J, Finney O, Mendoza J, Butcher GA, Andrews L, Hill AV, Gilbert SC, Basáñez M-G. 2007. Progression of Plasmodium berghei through Anopheles stephensi is density-dependent. PLoS Pathog 3:e195. doi: 10.1371/journal.ppat.0030195. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 30.Coppi A, Natarajan R, Pradel G, Bennett BL, James ER, Roggero MA, Corradin G, Persson C, Tewari R, Sinnis P. 2011. The malaria circumsporozoite protein has two functional domains, each with distinct roles as sporozoites journey from mosquito to mammalian host. J Exp Med 208:341–356. doi: 10.1084/jem.20101488. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 31.Herrera R, Anderson C, Kumar K, Molina-Cruz A, Nguyen V, Burkhardt M, Reiter K, Shimp R Jr, Howard RF, Srinivasan P, Nold MJ, Ragheb D, Shi L, DeCotiis M, Aebig J, Lambert L, Rausch KM, Muratova O, Jin A, Reed SG, Sinnis P, Barillas-Mury C, Duffy PE, MacDonald NJ, Narum DL. 2015. Reversible conformational change in the Plasmodium falciparum circumsporozoite protein masks its adhesion domains. Infect Immun 83:3771–3780. doi: 10.1128/IAI.02676-14. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 32.Golenda CF, Starkweather WH, Wirtz RA. 1990. The distribution of circumsporozoite protein (CS) in Anopheles stephensi mosquitoes infected with Plasmodium falciparum malaria. J Histochem Cytochem 38:475–481. doi: 10.1177/38.4.2181019. [DOI] [PubMed] [Google Scholar]
- 33.Myung JM, Marshall P, Sinnis P. 2004. The Plasmodium circumsporozoite protein is involved in mosquito salivary gland invasion by sporozoites. Mol Biochem Parasitol 133:53–59. doi: 10.1016/j.molbiopara.2003.09.002. [DOI] [PubMed] [Google Scholar]
- 34.Ghosh AK, Devenport M, Jethwaney D, Kalume DE, Pandey A, Anderson VE, Sultan AA, Kumar N, Jacobs-Lorena M. 2009. Malaria parasite invasion of the mosquito salivary gland requires interaction between the Plasmodium TRAP and the Anopheles saglin proteins. PLoS Pathog 5:e1000265. doi: 10.1371/journal.ppat.1000265. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 35.Ejigiri I, Ragheb DR, Pino P, Coppi A, Bennett BL, Soldati-Favre D, Sinnis P. 2012. Shedding of TRAP by a rhomboid protease from the malaria sporozoite surface is essential for gliding motility and sporozoite infectivity. PLoS Pathog 8:e1002725. doi: 10.1371/journal.ppat.1002725. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 36.Battista A, Frischknecht F, Schwarz US. 2014. Geometrical model for malaria parasite migration in structured environments. Phys Rev E Stat Nonlin Soft Matter Phys 90:042720. doi: 10.1103/PhysRevE.90.042720. [DOI] [PubMed] [Google Scholar]
- 37.Boysen KE, Matuschewski K. 2013. Inhibitor of cysteine proteases is critical for motility and infectivity of Plasmodium. mBio 4:e00874-13. doi: 10.1128/mBio.00874-13. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 38.Touray MG, Seeley DC, Miller LH. 1994. Plasmodium gallinaceum: differential lysis of two developmental stages of malaria sporozoites by the alternative pathway of complement. Exp Parasitol 78:294–301. doi: 10.1006/expr.1994.1031. [DOI] [PubMed] [Google Scholar]
- 39.Posthuma G, Meis JF, Verhave JP, Hollingdale MR, Ponnudurai T, Meuwissen JH, Geuze HJ. 1988. Immunogold localization of circumsporozoite protein of the malaria parasite Plasmodium falciparum during sporogony in Anopheles stephensi midguts. Eur J Cell Biol 46:18–24. [PubMed] [Google Scholar]
- 40.van de Sand C, Horstmann S, Schmidt A, Sturm A, Bolte S, Krueger A, Lütgehetmann M, Pollok J-M, Libert C, Heussler VT. 2005. The liver stage of Plasmodium berghei inhibits host cell apoptosis. Mol Microbiol 58:731–742. doi: 10.1111/j.1365-2958.2005.04888.x. [DOI] [PubMed] [Google Scholar]
- 41.Singh AP, Buscaglia CA, Wang Q, Levay A, Nussenzweig DR, Walker JR, Winzeler EA, Fujii H, Fontoura BMA, Nussenzweig V. 2007. Plasmodium circumsporozoite protein promotes the development of the liver stages of the parasite. Cell 131:492–504. doi: 10.1016/j.cell.2007.09.013. [DOI] [PubMed] [Google Scholar]
- 42.Sato Y, Montagna GN, Matuschewski K. 2014. Plasmodium berghei sporozoites acquire virulence and immunogenicity during mosquito hemocoel transit. Infect Immun 82:1164–1172. doi: 10.1128/IAI.00758-13. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 43.Touray MG, Warburg A, Laughinghouse A, Krettli AU, Miller LH. 1992. Developmentally regulated infectivity of malaria sporozoites for mosquito salivary glands and the vertebrate host. J Exp Med 175:1607–1612. doi: 10.1084/jem.175.6.1607. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 44.Aly AS, Vaughan AM, Kappe SH. 2009. Malaria parasite development in the mosquito and infection of the mammalian host. Annu Rev Microbiol 63:195–221. doi: 10.1146/annurev.micro.091208.073403. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 45.Akaki M, Dvorak JA. 2005. A chemotactic response facilitates mosquito salivary gland infection by malaria sporozoites. J Exp Biol 208:3211–3218. doi: 10.1242/jeb.01756. [DOI] [PubMed] [Google Scholar]
- 46.Gerberg EJ, Barnard DR, Ward RA. 1994. Manual for mosquito rearing and experimental techniques. American Mosquito Control Association, Inc, Lake Charles, Louisiana. [Google Scholar]
- 47.Xu X, Dong Y, Abraham EG, Kocan A, Srinivasan P, Ghosh AK, Sinden RE, Ribeiro JM, Jacobs-Lorena M, Kafatos FC, Dimopoulos G. 2005. Transcriptome analysis of Anopheles stephensi-Plasmodium berghei interactions. Mol Biochem Parasitol 142:76–87. doi: 10.1016/j.molbiopara.2005.02.013. [DOI] [PubMed] [Google Scholar]
- 48.Beaudoin RL, Strome CPA, Tubergen TA. 1974. Plasmodium berghei berghei: ectopic development of the ANKA strain in Anopheles stephensi. Exp Parasitol 36:189–201. doi: 10.1016/0014-4894(74)90058-7. [DOI] [PubMed] [Google Scholar]
- 49.Janse CJ, Franke-Fayard B, Waters AP. 2006. Selection by flow-sorting of genetically transformed, GFP-expressing blood stages of the rodent malaria parasite, Plasmodium berghei. Nat Protoc 1:614. doi: 10.1038/nprot.2006.88. [DOI] [PubMed] [Google Scholar]
- 50.Hliscs M, Nahar C, Frischknecht F, Matuschewski K. 2013. Expression profiling of Plasmodium berghei HSP70 genes for generation of bright red fluorescent parasites. PLoS One 8:e72771. doi: 10.1371/journal.pone.0072771. [DOI] [PMC free article] [PubMed] [Google Scholar]
Associated Data
This section collects any data citations, data availability statements, or supplementary materials included in this article.