Figure 4.

SOX4 reverses the suppressive effects of miR-130a on the EMT process and invasion of radioresistant cells. (A) Morphological changes in SOX4-overexpressing cells were compared with control cells with bright-phase microscopy. The images were captured with 200× magnification. (B-D) Expression of EMT markers including E-cadherin, N-cadherin, vimentin and ZO-1, was detected with immunofluorescence confocal microscopy (B), western blotting (C) and real time PCR (D). The data were normalized with cyclophilin as the internal control for real time PCR. Scale bar: 20 μm. (E) Representative images showing the invasive activity of SNU70RR-miR-130a/SOX4 cells were captured after 48 h of irradiation treatment. The invaded cells were stained with crystal violet. The graph shows the fold difference of invasive cells compared with control cells from five different fields of three independent experiments. (F) Rectal cancer cells were seeded into six well plates and irradiated with indicated doses. After 14 days, the cells were fixed and stained with crystal violet. The images were captured and showed colony growth in four rectal cancer cells. Percentage of survived cells was calculated by comparing with untreated control. (_⁎⁎P < .01).