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. 2019 Jul 9;9(17):4909–4922. doi: 10.7150/thno.33758

Figure 2.

Figure 2

SMAC-mimetics displace survivin from XIAP. (A) SH-EP cells were treated for the times indicated with 20 µM LCL161 or TL32711 respectively. Cells lysates were subjected to immunoblot analyses for cIAP1, cIAP2, XIAP and survivin. GAPDH served as loading control. SH-EP/Ctr and SH-EP/Surv (Surv) cells were treated with 10 µM LCL161 (B) or 10 µM TL32711 (C) for 2 hours. Cell lysates were subjected to immunoprecipitation for both anti-survivin and IgG control (upper panel) or anti-XIAP and IgG control (lower panel). Input lysates and precipitates were subjected to immunoblot analyses with antibodies directed against survivin and XIAP. α-Tubulin served as loading control.