Figure 2.

Binding specificity and endocytosis assay of Zapadcine-1. (A). Jurkat E6-1, J.gamma1, A3, BALL-1, THP-1, Reh of acute lymphocyte leukemia cells and NCI-H1975 and MSTO-211H lung cancer cells were incubated with Zapadcine-1 or anti-human IgG antibody. Cell-associated fluorescence was determined by FACS. (B) Approximately 5×10⁴ cells of MSTO-211H were incubated with 20 µg/mL of FITC-labeled Zapadcine-1 (green) and 1.0 mL of lysosome specific dye of Lyso-Tracker Red. Images were captured by the laser scanning confocal microscopy.