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. Author manuscript; available in PMC: 2019 Aug 13.
Published in final edited form as: Nature. 2017 Mar 1;544(7649):196–201. doi: 10.1038/nature21393

Figure 3. Mediator inter-module contacts (Med14 density segmented from the SpMED cryo-EM map is shown in semi-transparent gray in panels b-d).

Figure 3.

a, Contacts between Med14 and subunits in the Head, Middle and Tail modules. b, Med14-Med17 jaw interface. Selected Med17 residues labeled for reference. c, Contacts between Med14 and Head subunits Med6 and Med20. d, Med14-Middle interface. The N-terminal portion of Med14’s RM1 interacts with portions of Med4, Med7, Med9, and Med21, all of which converge near the center of the Middle module. The N-terminal portion of Med17 also contributes to the Med14-Middle interface. e, Knob structure. The knob’s central core is formed by Med31 (in red). The Med14 KID and Med17N domains cross module boundaries to converge on the knob, whose intricate structure includes elements from the Head, Middle, and Med14. Densities corresponding to Med14 KID and to Med17N were segmented from the SpMED cryo-EM map and are shown in semi-transparent orange and magenta, respectively.