FIG 2.

Inducible, ectopic expression of WT mtrR represses expression of mtrCDE and other MtrR-regulated genes. (A) Western blot analyses of whole-cell lysates of strains FA19, H041, and SC4 (with or without IPTG) probed for MtrE and MtrR. (B) Summary of fold changes of expression levels of representative antibiotic resistance genes in SC4 as the ratios of qRT-PCR results from RNA extracted from IPTG-treated versus -untreated cultures; specific values and statistical significance values are provided in Table S1. Asterisks indicate statistically significant results.