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Complementation of the wspA Δ285-298 mutation. wspA was complemented in representative RSCVs by replacing wspA Δ285-298 with the wild-type allele on the genome. RSCV-2 was selected as a representative RSCV with the wspA driver mutation alone. RSCV-1 and RSCV-40 have the wspA driver mutation as well as ΔpslE-pslO and wspD secondary mutations, respectively. Parent and complemented RSCVs (labeled) were grown on VBMM, and colony morphologies were assessed. Bars, 2 mm.
