Figure 2.
RBC post-thaw recovery [defined as (1 – haemolysis (×100))] with cryoprotective polymers. (a) RBC frozen rapidly to −196 °C/thaw 45 °C. (b) Effect of prefreezing incubation time and temperature on post-thaw recovery with 100 mg mL–1P2. Incubation time (0, 15, 30 min, 24 h), incubation temperature (4 or 23 °C), and freezing conditions [directly submerged (direct) or in vapor (vapor) of liq. N2]. All error bars represent ±SEM from a minimum of three repeats.