Table 1. Effect of KMT2D silencing on the lipidomic profile of pancreatic cancer cells.
Quantitative LC-MS data of FAs and total cholesterol in MIA PaCa-2 cells pretreated with siC#1 or siKMT2D#2. The top 3 lipids, most significantly elevated by KMT2D reduction, are shown in bold. Statistical analyses were performed using one-way ANOVA.
| Lipid | siC#1 (ng) per 50,000 cells |
siKMT2D#2 (ng) per 50,000 cells |
% percentage change | P value |
|---|---|---|---|---|
| Docosatrienoic acid | .6 | 1.5 | +150 | .00482 |
| Docosadienoic acid | .35 | 1 | +186 | .00971 |
| Docosatretraenoic acid | 1.425 | 3.5 | +150 | .01084 |
| Nervonic acid | 4.1 | 10.1 | +146.3 | .00217 |
| Eicosadienoic acid | 2.825 | 6.55 | +131.8 | .00557 |
| Dihomo-g-linolenic acid | 1.3 | 2.925 | +125 | .0075 |
| Eicosapentaenoic acid | 8 | 17.075 | +113.4 | .19345 |
| Eicosenoic acid | 32.675 | 68.675 | +110.2 | .00116 |
| Docosapentaenoic acid (n=3) | 4.55 | 9.1 | +100 | .000229 |
| Docosenoic acid | 3.65 | 6.825 | +87 | .00364 |
| Docosahexaenoic acid | 4.675 | 8.2 | +75.4 | .02643 |
| Arachidonic acid | 8.933 | 13.825 | +54.8 | .009 |
| g-Linolenicacid | 8.325 | 12.175 | +46.2 | .16032 |
| Oleic acid | 715.6 | 1049.775 | +46.7 | .01402 |
| a-Linolenic acid | 4.1 | 5.9 | +43.9 | .16846 |
| Stearic acid | 2563.2 | 3667.875 | +43.1 | .11082 |
| Linoleic acid | 23.7 | 33.575 | +41.7 | .07341 |
| Hexacosanoic acid | 7.05 | 9.95 | +41.1 | .13026 |
| Cholesterol | 359.25 | 474.75 | +32.2 | .04811 |
| Dodecanoic acid | 82.25 | 108.1 | +31.4 | .0013 |
| Palmitoleic acid | 118.775 | 148.575 | +25.1 | .26513 |
| Lignoceric acid | 25.375 | 30.825 | +21.5 | .21665 |
| Palmitic acid | 7676 | 8941.525 | +16.5 | .05763 |
| Docosanoic (Behenic acid) | 9.575 | 10.475 | +9.4 | .38668 |
| Heptadecenoic acid | 71.55 | 78.075 | +9.1 | .10477 |
| Pentadecanoic | 486.375 | 524.125 | +7.8 | .27158 |
| Heptadecanoic acid | 91.15 | 96.85 | +6.3 | .35989 |
| Arachidic acid | 20.55 | 21.65 | +5.4 | .5251 |
| Myristic acid | 459.625 | 480.375 | +4.5 | .08533 |