Table 3.

Effect of various inhibitors and metal ions on protease activity. The non-treated enzyme was considered as control (100%).

Inhibitor/Metal ion	Relative activity (%)
	2mM	5mM
Control	100 ± 2.16	100 ± 2.16
PMSF	5 ± 0.11	0 ± 0.0
EDTA	111 ± 2.16	102 ± 1.90
HgCl2	70 ± 1.23	64 ± 1.24
Urea	125 ± 1.27	120 ± 2.43
pCMB	100 ± 1.98	95 ± 1.86
DTNB	80 ± 1.42	74 ± 1.72
2-ME	126 ± 2.54	143 ± 2.18
FeSO4	84 ± 1.86	98 ± 2.67
CaCl2	102 ± 2.43	105 ± 2.55
KCl	99 ± 2.54	101 ± 2.34
NaCl	68 ± 1.64	70 ± 1.62
MgSO4	92 ± 2.0	94 ± 1.78
MnCl2	91 ± 2.03	86 ± 1.71
CuSO4	110 ± 2.64	96 ± 2.08
HgCl2	73 ± 1.54	68 ± 1.45
ZnCl2	94 ± 1.78	83 ± 1.88
NiCl2	93 ± 2.04	84 ± 1.78