Figure 6.
GFP-TMEM88 Localizing in EEA1+ Digitonin-Resistant Body (MVB) Enhances the MVB Co-localization of CA-LRP6-mPlum
(A) 293T cells were transfected with plasmid vectors expressing GFP-TMEM88 or GFP and CA-LRP6-mPlum; 24 h after transfection, the cells were fixed and the nucleus was stained with DAPI.
(B) Quantification of (A). The localization of CA-LRP6-mPlum was scored as either being present in cytoplasmic vesicles or in perinuclear MVB. ∗∗∗Chi squared p value: 1.14 × 10−13. For GFP, N = 46. For GFP-TMEM88, N = 53.
(C) 293T DVL TKO cells were transfected with plasmid vectors expressing GFP-TMEM88 or GFP and CA-LRP6-mPlum; 48 h after transfection, the cells were stained with DAPI and live imaged.
(D) Quantification of (C). The localization of CA-LRP6-mPlum was scored as either being present in cytoplasmic vesicles or in perinuclear MVB. ∗∗∗Chi squared p value: 4.39 × 10−9. For GFP, N = 40. For GFP-TMEM88, N = 60.