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. Author manuscript; available in PMC: 2020 Mar 8.
Published in final edited form as: Science. 2019 Mar 8;363(6431):eaat4042. doi: 10.1126/science.aat4042

Fig 6. MATE is CDC42-dependent and dynamin-dependent.

Fig 6.

(A, B) Quantification of active CDC42-GTP and CDC42EP1 expression in IECs isolated from terminal ileum of C57BL/6J mice 10 days after colonization with SFB. CDC42-GTP levels in (A) were normalized to total CDC42 and CDC42EP1 levels in (B) were normalized to β-actin. Data points represent individual animals. (C-F) MATE is CDC42-dependent and dynamin-dependent. NSG mice were colonized with SFB for at least one month. After establishment of persistent colonization, chemical inhibitors were introduced into externalized terminal ileum intestinal loops of live animals by perfusion in vivo as described in Methods. (C) Representative electron tomograms of SFB-IEC synapses. All scale bars are 200 nm. (D-F) Quantification of MATE vesicle morphology. Data points represent individual MATE vesicles. All error bars, standard deviation. Statistics, (A, D-F) unpaired two-tailed t test, (B) Mann-Whitney-Wilcoxon test.