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Localization of core complex macromolecular components to the various regions of the cryo-EM structure. A. Immunolocalization of the N-terminus of TraF/VirB10 by negative stain EM. Top panel: representative class averages are shown. Lower panel: same as top panel but with additional density due to anti-His6 antibodies colored in red. B. Localization of TraO/VirB9 (blue) and TraF/VirB10 (red) within the cryo-EM structure of the wild-type core complex.
