Table 1.
Overview of sampling sites, number of analyzed specimens per site, DNA extraction method used (SaPr = salt precipitation and/or CH = Chelex), and morphological identification level.
| Site | Stream | Coordinates [N/E] | Geographical region | Year | Number of samples | Extraction method | Identification level |
| Svb | Silvertbach | 51.644583 7.230139 | North Rhine-Westphalia | 2017 | 7 | SaPr | species |
| D | Diemel | 51.420806 8.808667 | North Rhine-Westphalia | 2017 | 7 | SaPr | species |
| V | Volme | 51.241611 7.531167 | North Rhine-Westphalia | 2017 | 2 | SaPr | species |
| 1OW | Tributary Lüderbach | 50.475897 9.295219 | Hesse | 2018 | 5 + 1 O. monedula | CH | genus |
| 2KS | Tributary Rammholzer Wasser | 50.331768 9.619321 | Hesse | 2018 | 5 + 3 O. monedula | all samples with CH, 2 O. monedula also with SaPr | genus |
| 3OE | Tributary Lohrbach | 50.116317 9.462612 | Hesse | 2018 | 7 | all samples with CH, 2 Sericostoma also with SaPr | genus |
| 2OS | Schmale Sinn | 50.33576 9.696705 | Hesse | 2018 | 6 | all samples with CH, 2 Sericostoma also with SaPr | genus |
| Han | Hannebecke | 51.30635 8.41007 | North Rhine-Westphalia | 2014 | 1 | SaPr | species |
| Val | Valme | 51.31553 8.40354 | North Rhine-Westphalia | 2014 | 2 | SaPr | species |
| Nie | Nier | 51.31380 8.35892 | North Rhine-Westphalia | 2014 | 4 | SaPr | species |
| Bra | Brabecke | 51.29762 8.40043 | North Rhine-Westphalia | 2014 | 3 | SaPr | species |
| Nes | Nesselbach | 51.17494 8.41878 | North Rhine-Westphalia | 2014 | 3 | SaPr | species |
| Roe | Röhr | 51.27247 8.05820 | North Rhine-Westphalia | 2014 | 7 + 1 hybrid | SaPr | species |
| Sch | Schwarze Ahe | 51.20499 7.72184 | North Rhine-Westphalia | 2014 | 3 | SaPr | species |
| Kru | Krummenau | 51.07383 7.71277 | North Rhine-Westphalia | 2014 | 9 + 2 hybrids | SaPr | species |
| Sor | Sorpe | 51.19883 8.42851 | North Rhine-Westphalia | 2013 | 3 | SaPr | species |
| Sil | Silberbach | 51.03180 8.05244 | North Rhine-Westphalia | 2014 | 3 | SaPr | species |