Table 1. Sequences of primers used in RT-qPCR.
Primer | Sequence (5′–3′) |
---|---|
PDH-F | TCAACTACCTGGTGCTTCG |
PDH-R | CATCTCCAAATGCCCTAA |
sdhA-F | ATTAACAGTCAAGGCGAAAG |
sdhA-R | ACAACCAGGTCCAAGAGC |
GPx-F | CCAGTCGGTGTATGCCTTCT |
GPx-R | GATGTCAGGCTCGATGTCAA |
GR-F | GCTGATTAAAGCTTTCCAAGTTGTG |
GR-R | GTAAAGCTCGAGGAATAGGTCTTCAC |
GOT-F | ACTCAAGGAGAAGCGGGTAG |
GOT-R | ACAGGCGTGGAGGACAAC |
CS-F | CGTTTCCGAGGCTTTAGT |
CS-R | ACAAGGTAGCTTTGCGATT |
IDH-F | CACTACCGCATGTACCAGAAAGG |
IDH-R | TCTGGTCCAGGCAAAAATGG |
LDH-F | TGTGCCTGTATGGAGTGG |
LDH-R | TTATTCCGTAAAGACCCT |
PK-F | GCACGCCAAGTACAACACC |
PK-R | CACGCTCCCACATTCCATA |
β-actin-F | GGGACCTGACTGACTACCTC |
β-actin-R | TCATACTCCTGCTTGCTGAT |
Note:
PDH, pyruvate dehydrogenase; sdhA, succinate dehydrogenase; GPx, glutathione peroxidase; GR, glutathione reductase; GOT, glutamic oxaloacetic transaminase; CS, citrate synthase; IDH, isocitrate dehydrogenase; LDH, lactic dehydrogenase; PK, pyruvate kinase.