Table 2.
Linearity and sensitivity of UPLC-MS/MS assay for ophiopogonins in fermented broth of human gut microbiota.
| Analyte | Calibration Curve | R 2 a | Linear Range (μM) |
LLOQ b
(μM) |
LOD c
(μM) |
|---|---|---|---|---|---|
| Ophiopogonin D | y = 0.4461x − 0.0870 | 0.9986 | 0.24–60 | 0.24 | 0.07 |
| Ophiopogonin D’ | y = 0.4688x − 0.0442 | 0.9976 | 0.24–60 | 0.24 | 0.07 |
| Ruscogenin | y = 0.3848x + 0.1414 | 0.9985 | 0.24–60 | 0.24 | 0.07 |
| Diosgenin | y = 0.3767x + 0.1790 | 0.9977 | 0.24–60 | 0.24 | 0.07 |
aR2, coefficient of determination; b LLOQ: lower limit of quantification; c LOD: limit of detection.