Table 3.
The intra-day and inter-day precision and accuracy for UPLC-MS/MS assay of ophiopogonins in fermented broth of human gut microbiota.
| Analyte | Spiked (μM) | Intra-Day (n = 5) | Inter-Day (n = 15) | ||||
|---|---|---|---|---|---|---|---|
| Measured (μM) | Precision (RSD%) | Accuracy (RE%) | Measured (μM) | Precision (RSD%) | Accuracy (RE%) | ||
| Ophiopogonin D | 0.60 | 0.62 | 3.80 | 3.43 | 0.59 | 11.87 | −1.79 |
| 3.00 | 2.89 | 8.09 | −3.61 | 3.06 | 13.63 | 2.10 | |
| 30.0 | 29.2 | 10.07 | −2.57 | 31.6 | 10.31 | 5.43 | |
| Ophiopogonin D’ | 0.6 | 0.59 | 12.05 | −2.25 | 0.65 | 9.46 | 8.79 |
| 3.0 | 3.11 | 12.88 | 3.60 | 3.24 | 11.51 | 7.91 | |
| 30.0 | 30.2 | 14.87 | 0.68 | 31.9 | 8.09 | 6.30 | |
| Ruscogenin | 0.6 | 0.65 | 5.95 | 9.12 | 0.65 | 3.96 | 7.94 |
| 3.0 | 3.20 | 4.94 | 6.68 | 3.20 | 6.36 | 6.91 | |
| 30.0 | 31.8 | 12.39 | 6.17 | 33.4 | 12.67 | 11.51 | |
| Diosgenin | 0.6 | 0.65 | 6.14 | 8.95 | 0.62 | 8.68 | 4.51 |
| 3.0 | 3.13 | 3.74 | 4.43 | 3.07 | 8.78 | 2.40 | |
| 30.0 | 30.19 | 8.04 | 0.62 | 31.6 | 8.45 | 5.19 | |