Figure 1.

In vitro phosphorylation of Y328 and Y331 on histone deacetylase 3 (HDAC3). (A) Prediction of putative phosphorylation sites at tyrosine residues on HDAC3. Putative tyrosine phosphorylation residues on HDAC3 were predicted using an artificial neural network predictor of phosphorylation sites, the Net Phos3.1 (http://cbs.dtu.dk) program. The proto-oncogene tyrosine-protein kinase (c-Src)-mediated putative phosphorylation residues are highlighted in red. (B) HDAC3 is phosphorylated by c-Src. In vitro kinase assays were performed with recombinant c-Src and the indicated glutathione (GST)-fused HDAC3 proteins. The samples were processed for SDS-PAGE and subsequently visualized by autoradiography. CBB, Coomassie blue staining.