. 2019 Aug 20;8(8):937. doi: 10.3390/cells8080937

Table 1.

ACCEPT gates used for the automated enumeration of leukocytes, leukocyte-derived Extracellular Vesicles (ldEVs), platelets, and red blood cells in blood A with EpCAM enrichment and B without EpCAM enrichment.

		A. EpCAM Enrichment		B. No Enrichment
Leukocytes	DAPI/Hoechst ^a	Mean Intensity	>30	Mean Intensity	>30
		Max Intensity	>50	Max Intensity	>50
		Size	>16 μm²	Size	>16 μm²
	CD45	Mean Intensity	>30	Mean Intensity	>30
		Max Intensity	>50	Max Intensity	>50
				Size	≤400 μm²
	CK	Standard Deviation	≤5	n/a ^b
	CD61	n/a ^b		Standard Deviation	≤5
	CD235a	n/a ^b		Standard Deviation	≤5
	Extra channel	Standard Deviation	≤5	Standard Deviation	≤5
ldEVs	DAPI/Hoechst ^a	Standard Deviation	≤5	Standard Deviation	≤5
	CD45	Mean Intensity	>30	Mean Intensity	>30
		Max Intensity	>50	Max Intensity	>50
		Perimeter	>5 pixels	Perimeter	>5 pixels
		Size	≤150	Size	≤150 μm²
		Eccentricity	≤0.85	Eccentricity	≤0.85
	CK	Standard Deviation	≤5	n/a ^b
	CD61	n/a ^b		Standard Deviation	≤5
	CD235a	n/a ^b		Standard Deviation	≤5
	Extra channel	Standard Deviation	≤5	Standard Deviation	≤5
Platelets	CD45	n/a ^b		Standard Deviation	≤5
	CD61			Mean Intensity	>30
				Max Intensity	>50
				Perimeter	>5 pixels
				Size	≤150 μm²
				Eccentricity	≤0.85
	CD235a			Standard Deviation	≤5
	CD235a			Standard Deviation	≤5
	Extra Channel			Standard Deviation	≤5
Red blood cells	Hoechst	n/a ^b		Standard Deviation	≤5
	CD45			Standard Deviation	≤5
	CD61			Standard Deviation	≤5
	CD235a			Mean Intensity	>30
				Max Intensity	>50
				Perimeter	>5 pixels
	Extra Channel			Standard Deviation	≤5

^a DAPI was used in EpCAM-enriched blood and Hoechst in the blood without pre-enrichment, ^b n/a: not applicable.