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(A) RT-PCR showing that the female-specific BgDsx exon 3/4 junction (Figure 2B,C) is absent in dsBgTra females (B) RT-PCR showing that the male-specific BgDsx exon 3/5 junction (Figure 2B) is present in dsBgTra females. dsBgTra females do not express any of the female-specific BgDsx isoforms. (C) qPCR shows complete absence of the female-specific BgDsx exon 3/4 junction in dsBgTra females.
