Table 1.
E. coli isolates from 20 healthy subjects. The strains were genotyped by ERIC-PCR, RAPD-PCR, and PFGE analysis. The relative abundance represents the ratio of each strain within the set of E. coli clones analyzed. The charge of the viable cells has been calculated by multiplying the total E. coli count and the relative amount. Phlylotyping was done according to Clermont et al. 2013 [17]. The presence of β-glucuronidase was confirmed by growth of blue colonies on the HCCA medium. The performance of isolates as recipients in conjugation experiments was assessed only in β-glucuronidase negative strains. Biofilm formation was tested on LBWS and M9glu media. Curli and cellulose production was determined.
| E. coli Strain | Genotyping and Phyloptyping | Phenotype Assays | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ERIC-PCR | RAPD-PCR Profile | XbaI-PFGE Profile | Phylo-Group | β-glu | Conju-Gation | Biofilm | Curli | Cellulose | ||||
| Profile | Relative Abundance | Log (cfu/g) | LBWS | M9 | ||||||||
| 01.01 | E01 | 100% | 7.56 | R01 | P01 | B1 | + | − | − | − | − | |
| 02.03 | E02 | 8% | 5.22 | R02 | P02 | B2 | + | − | − | − | − | |
| 02.16 | E03 | 92% | 6.28 | R03 | P03 | F | + | + | − | + | + | |
| 03.25 | E04 | 99% | 7.52 | R04 | P04 | B2 | + | + | − | − | − | |
| 03.73 | E05 | 1% | 5.53 | R03 | P05 | A | + | + | + | − | − | |
| 04.05 | E06 | 5% | 4.22 | R05 | P06 | A | + | + | − | + | − | |
| 04.06 | E07 | 70% | 5.37 | R06 | P07 | B2 | + | − | − | − | − | |
| 04.10 | E08 | 25% | 4.92 | R07 | P08 | E | + | − | − | + | + | |
| 05.20 | E07 | 96% | 7.78 | R02 | P09 | B2 | + | − | − | − | + | |
| 05.47 | E09 | 4% | 6.40 | R08 | P10 | B1 | − | + | − | − | − | − |
| 06.16 | E07 | 100% | 7.28 | R07 | P11 | B2 | + | − | − | − | − | |
| 07.09 | E10 | 100% | 6.45 | R04 | P12 | D | + | + | − | + | − | |
| 08.01 | E11 | 18% | 4.91 | R09 | P12 | D | + | + | − | + | − | |
| 08.02 | E12 | 5% | 4.37 | R10 | P13 | A | + | − | + | − | − | |
| 08.10 | E13 | 5% | 4.37 | R11 | P14 | A | + | + | + | + | − | |
| 08.27 | E14 | 36% | 5.20 | R11 | P15 | B1 | − | + | − | + | − | − |
| 08.57 | E15 | 34% | 5.18 | R12 | P16 | F | − | + | − | − | − | − |
| 08.109 | E16 | 2% | 3.86 | R13 | P17 | F | − | + | − | − | − | − |
| 09.13 | E01 | 92% | 7.09 | R14 | P18 | C | + | + | − | + | + | |
| 09.21 | E17 | 4% | 5.73 | R12 | P19 | F | + | − | − | − | − | |
| 09.42 | E07 | 4% | 5.73 | R12 | P09 | B2 | + | − | − | − | − | |
| 10.17 | E04 | 100% | 7.62 | R12 | P04 | B2 | + | + | − | − | − | |
| 11.02 | E18 | 50% | 7.67 | R15 | P20 | A | + | − | − | − | − | |
| 11.04 | E19 | 9% | 6.91 | R10 | P21 | A | + | − | − | − | − | |
| 11.06 | E07 | 37% | 7.54 | R16 | P22 | B2 | + | − | − | − | − | |
| 11.16 | E20 | 4% | 6.57 | R17 | P23 | B2 | + | − | − | − | − | |
| 12.04 | E13 | 71% | 7.43 | R18 | P24 | B2 | + | − | − | + | − | |
| 12.09 | E11 | 29% | 7.04 | R18 | P25 | D | + | − | − | − | − | |
| 13.01 | E01 | 3% | 4.98 | R19 | P26 | B1 | + | − | − | + | − | |
| 13.03 | E11 | 1% | 4.47 | R11 | P27 | D | + | − | − | + | + | |
| 13.20 | E21 | 97% | 6.46 | R13 | P28 | F | − | − | − | − | − | − |
| 14.03 | E22 | 100% | 6.85 | R13 | P29 | E | + | − | − | + | − | |
| 15.01 | E07 | 9% | 6.45 | R13 | P30 | B2 | + | − | − | − | + | |
| 15.02 | E01 | 87% | 7.45 | R11 | P31 | B1 | + | + | − | + | + | |
| 15.13 | E11 | 4% | 6.15 | R20 | P32 | D | + | − | − | + | − | |
| 16.04 | E21 | 1% | 5.74 | R21 | P33 | F | + | − | − | − | − | |
| 16.15 | E01 | 1% | 5.74 | R22 | P34 | B1 | + | + | − | + | − | |
| 16.27 | E21 | 98% | 7.73 | R23 | P33 | F | − | − | − | − | − | − |
| 17.10 | E23 | 87% | 7.45 | R23 | P35 | A | + | − | − | − | − | |
| 17.18 | E07 | 13% | 6.63 | R24 | P36 | B2 | + | + | − | − | − | |
| 18.03 | E24 | 39% | 6.25 | R25 | P37 | B1 | + | − | + | − | − | |
| 18.09 | E13 | 41% | 6.28 | R25 | P24 | B2 | + | − | − | + | − | |
| 18.18 | E25 | 19% | 5.93 | R13 | P38 | E | + | − | − | − | + | |
| 18.50 | E26 | 1% | 4.70 | R21 | P39 | B1 | − | + | − | − | − | − |
| 19.02 | E07 | 100% | 7.19 | R14 | P40 | B2 | + | + | − | − | − | |
| 20.04 | E27 | 12% | 6.39 | R26 | P41 | B1 | + | − | − | + | − | |
| 20.17 | E28 | 5% | 6.01 | R12 | P42 | C | + | − | − | + | − | |
| 20.27 | E29 | 53% | 7.03 | R27 | P43 | E | + | + | − | + | + | |
| 20.50 | E30 | 22% | 6.65 | R04 | P44 | B1 | − | + | − | − | − | − |
| 20.51 | E31 | 4% | 5.91 | R06 | P44 | B1 | − | + | − | − | − | − |
| 20.71 | E09 | 4% | 5.91 | R06 | P44 | B1 | − | + | − | − | − | − |