Figure 2.

Mapping the ubiquitylation sites of Ent1. A scheme showing the approaches taken to identify the ubiquitylation sites of Ent1. S. cerevisiae and E. coli expression and purification systems described in the methods section. Purified proteins were subjected to western blot analysis with the indicated antibodies. The ubiquitylated lysine residues (Ks) identified by the mass spectrometry analysis are indicated.