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. 2019 Jul 25;9(8):306. doi: 10.3390/biom9080306

Figure 1.

Figure 1

AC treatment decreases the viability of CRC cells by inducing apoptosis. (A) Cell viability assay demonstrates that AC is effectively suppressing the cellular viability in CRC cells. The IC50 values of AC in all three cells (SW480, SW620 and HCT116) are indicated. (B) AC effectively suppresses colony-forming ability. *** p < 0.001. (C) Colon cancer cell lines were treated with AC (100 µg/mL) for 24 h and then analyzed for apoptosis by flow cytometer for Annexin-V+ and PI+ stained cells. AC significantly induces apoptosis in CRC cells (SW480, SW620 and HCT116). Number in the box indicates the percentage of Annexin-V+ cells (control versus AC treatment, left and right respectively). (D) Western blots of the whole-cell lysates from AC treated colon cancer cells showed a significantly increased level of cleaved PARP (pro-apoptotic marker).