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. 2019 Aug 8;11(8):730. doi: 10.3390/v11080730

Figure 1.

Figure 1

cDCs and pDCs in BM can produce interferon-β during RSV infection. (A) Flow cytometric gating strategy for cells. Representative dot plots showing cDCs in the CD11chiSiglec-H−MHC-IIhi and pDCs in the CD11bloCD11cintSiglec-H+Ly6C+ populations, gated on CD45.2+DAPI−. (B) BM cells from interferon-β/YFP reporter mice were infected with RSV at a multiplicity of infection of 3 or with 2.5 μg/mL CpG2216. Cells were harvested 18 h after stimulation and analyzed for the expression of interferon-β by flow cytometry. The remaining cells (remnants) were defined as the population of CD11b−CD11c−Ly6C− cells gated on DAPI−. (C–E) BM cells were infected with RSV at a multiplicity of infection value of 3 or with 2.5 μg/mL CpG2216. The supernatant was collected 18 h after stimulation and analyzed for interferon-β (C), IL-6 (D), and IL-12p40 (E) by ELISA. The data are presented as the mean ± SEM and representative of three independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001 as calculated by the Student’s t test.