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The ESCRT-III complex proteins CHMP1-GFP and SEC31-GFP do not accumulate at the plasma membrane during fusion-induced lysis or after nystatin treatment. Subcellular localization of CHMP1-GFP (GN9-50) and SEC31-GFP (GN9-48) in untreated healthy germlings (A), during fusion failure (B), and during nystatin-induced cell lysis (C). Fusion points are indicated (arrows). Left images: DIC; right images: GFP. Bar, 5 µm.
