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. 2019 Jul 11;213(1):173–194. doi: 10.1534/genetics.119.302458

Figure 5.

Figure 5

MPK-1/ERK is hyperphosphorylated and required for elevated DNA damage-induced apoptosis in alg-2(ok304) mutant animals. (A) The mild ERK signaling loss-of-function alleles mpk-1(ga111) and mek-2(n1989) prevented MPK-1 phosphorylation in IR-treated (30 Gy) alg-2(ok304) mutant gonad loops. Germlines were dissected 2.5 hr post-treatment. Representative images were obtained from Z-stacks taken with a confocal microscope, germline loop regions are highlighted by dashed lines. Bars, 20 µm. (B) Quantification of WT, alg-2(ok304), alg-2(ok304);mpk-1(ga111), and alg-2(ok304);mek-2(n1989) mutant germlines displaying induced MPK-1 phosphorylation in the late-pachytene zone or the entire gonad loop at 2.5 hr post-IR. Phospho-MPK-1 signals were assessed at 10× and 40× magnification with a fluorescent microscope. Sample sizes are indicated. (C) The mpk-1(ga111) and mek-2(n1989) alleles alleviated DNA damage-induced germ cell apoptosis in alg-2(ok304) mutant animals. Apoptosis was scored 4 hr post-IR treatment (30 Gy). Representative graph from one out of three independent experiments, medians with 95% C.I.s are shown, a minimum of 15 germlines per genotype and condition were scored. **** P < 0.0001; Mann–Whitney nonparametric test. #, number; IR, ionizing radiation; WT, wild-type.