KEY RESOURCES TABLE
| Resource/Reagent | Source | Identifier |
|---|---|---|
| Experimental Models: Organisms/Strains | ||
| Strain (Drosophila melanogaster) 48A08AD (II), 66A01DBD (III) [L1 split-GAL4] |
Janelia Research Campus(Tuthill et al.,2013) | N/A |
| Strain (Drosophila melanogaster) 53G02AD (II), 29G11DBD (III) [L2 split-GAL4] | Janelia Research Campus(Tuthill et al.,2013) | N/A |
| Strain (Drosophila melanogaster) 64B03AD (II), 14B07DBD (III) [L3 split-GAL4] |
Janelia Research Campus(Tuthill et al.,2013) | N/A |
| Strain (Drosophila melanogaster) 31C06AD (II), 34G07DBD (III), [L4 split-GAL4] | Janelia Research Campus(Tuthill et al.,2013) | N/A |
| Strain (Drosophila melanogaster) 64D07AD (II), 37E10DBD (III) [L5 split-GAL4] |
Janelia Research Campus(Tuthill et al.,2013) | N/A |
| Strain (Drosophila melanogaster) 79C23S-GS-FRT-stop-FRT-smFPV5–2A-LexAVP16 | J. Peng (Peng et al., 2018) | N/A |
| Strain (Drosophila melanogaster) LexAop-myr::tdTomato (III) | Akin and Zipursky, 2016 | N/A |
| Strain (Drosophila melanogaster) UAS-Flp (II) | Bloomington Drosophila Stock Center | RRID:BDSC_4540 |
| Strain (Drosophila melanogaster) 27G05-FLPG5.PEST (attp5) | Janelia research campus (Peng et al., 2018) | N/A |
| Strain (Drosophila melanogaster) GMR-GAL4 (III) | Bloomington Drosophila Stock Center | RRID:BDSC_8121 |
| Strain (Drosophila melanogaster) 27G05-GAL4 (attp2) | Bloomington Drosophila Stock Center | RRID:BDSC_48703 |
| Strain (Drosophila melanogaster) UAS-β-RNAi (attp40) | Bloomington Drosophila Stock Center | RRID:BDSC_38310 |
| Strain (Drosophila melanogaster) 9B08GAL4 | (Pecot et al., 2013) | N/A |
| Strain (Drosophila melanogaster) DIP-β1−95 | This study | N/A |
| Strain (Drosophila melanogaster) DIP-γ1−67 | This study | N/A |
| Strain (Drosophila melanogaster) UAS-DIP-γ | This study | N/A |
| Strain (Drosophila melanogaster) UAS-DIP-ε | This study | N/A |
| Strain (Drosophila melanogaster) UAS-DIP-β | This study | N/A |
| Antibodies | ||
| Antibody Anti-V5 (mouse) 1:200 | Bio-Rad/AbD Serotec | Cat# MCA2892GA; RRID:AB_1658039 |
| Antibody Anti-DsRed (rabbit) 1:200 | Clontech Laboratories, Inc. | Cat# 632496; RRID:AB_10013483 |
| Antibody anti-chaoptin (mouse) 1:20 | Developmental Studies Hybridoma Bank | Cat# 24B10, RRID:AB_528161 |
| Antibody Anti-DIP-Beta (guinea pig) 1:300 | This study | N/A |
| Antibody Anti-DIP-Epsilon (rabbit) 1:500 | This study | N/A |
| Antibody Anti-DIP-Gamma (guinea pig) 1:400 | This study | N/A |
| Antibody Goat anti-Mouse IgG (H&L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 1:500 | Thermo Fisher Scientific | Cat# A11029 |
| Antibody Antibody, Alexa Fluor 647 1:500 | Thermo Fisher Scientific | Cat# A21245 |
| Antibody 647 Goat anti-Guinea Pig IgG (H&L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 1:500 | Thermo Fisher Scientific | Cat# A21450 |
| Software and Algorithms | ||
| Cartridge tracing algorithm | This paper | https://hms-idac.github.io/VoxelClassifier/ |
| MATLAB | Mathworks | https://www.mathworks.com/products/matlab.html |
| Prism | Graphpad | https://www.graphpad.com/scientific-software/prism/ |
| Fiji | Schindelin et al., 2012 | https://fiji.sc/ |
| R https://www.r-project.org/ | R core team (2013) | https://www.r-project.org/ |