Fig. 2.

Characterization of CD4⁺ T cell subpopulations in PBMC following RB51 immunological challenge of VPEAR- and RB51-vaccinated animals. (A) Top panels are representative dot plots of PBMC analyzed ex vivo showing FSC vs. SSC profile and gating strategy for singlet discrimination and viability. Bottom panels are representative dot plots showing staining profiles with and gating strategies for CD3, CD4, CD45RO, CCR7 and CD62L. Shown are the kinetics of (B) total CD4⁺ T cells, (C) CD4⁺ memory T cells (CD45RO⁺), (D) central memory CD4⁺ T cells (T_CM: CD45RO⁺ CCR7⁺ CD62L⁺), (E) effector memory CD4⁺ T cells (T_EM: CD45RO⁺ CCR7^- CD62L⁻), and (F) effector CD4⁺ T cells (T_eff: CD45RO⁻ CCR7^- CD62L⁻) in the peripheral blood of VPEAR- (black circles) and RB51-vaccinated (open circles) animals following RB51 immunological challenge or boost.