Summary
Graphdiyne (GDY) as an emerging 2D carbon-network nanomaterial possesses many fascinating properties that lead to numerous exciting applications, but the use of GDY and its derivatives in the antibacterial field has not yet been discovered. In this study, we first report on the use and evaluation of GDY and graphdiyne oxide (GDYO) as antibacterial agents and propose the antibacterial mechanisms of GDY-based nanomaterials. GDYO has been synthesized via the surface oxidation of GDY, and the antibacterial activity of GDYO has been compared with that of GDY through a series of antibacterial tests. Surprisingly, surface oxidation endowed inert GDY with superior antibacterial capability against two representative bacterial models: Escherichia coli and Staphylococcus aureus. Antibacterial mechanism experiments disclose that the antibacterial function of GDYO is a result of reactive oxygen species-dependent oxidation stress when a dispersed GDYO suspension has a direct contact with bacteria especially under visible light irradiation.
Subject Areas: Chemical Synthesis, Nanomaterials, Materials Characterization
Graphical Abstract
Highlights
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We report for the first time on the antibacterial behaviors of GDY and GDYO
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Surface of GDY was modified with hydrophilic functional groups
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Surface oxidation endows inert GDY with superior antibacterial capability
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GDYO shows ROS-dependent antibacterial action after direct contact with bacteria
Chemical Synthesis; Nanomaterials; Materials Characterization
Introduction
Bacterial infections pose an unprecedented global challenge to public health, especially with the emergence of antibiotic-resistant pathogens that have developed through the overuse or misuse of antibiotics (Simpson et al., 2009, Han et al., 2011, Chambers and DeLeo, 2009; Rodriguez et al., 2014, Wellington et al., 2013). The continuing emergence and global spread of antibiotic-resistant bacterial strains have generated great interest, from bench researchers in academic laboratories to clinical trials to explore new antibacterial agents that act differently from traditional antibiotics to combat harmful pathogen-associated diseases (Li et al., 2018a, Bunders et al., 2011, Huang et al., 2015a, Sikandar et al., 2018). Compared with traditional antibiotics, antibacterial nanomaterials are not prone to generate bacterial resistance because they not only act through multiple antibacterial mechanisms simultaneously but also have good membrane permeability owing to their small sizes (Li et al., 2018b, Hemeg, 2017, Rigo et al., 2018). To date, various advanced nanomaterials—including metals and metal oxides, MoS2, MXenes, and carbon nanomaterials, among others—have demonstrated great potential for managing bacterial infections (Tang and Zheng, 2018, Djurišić et al., 2014, Yang et al., 2014, Rasool et al., 2016, Lu et al., 2017). Of the antibacterial nanomaterials that effectively kill pathogenic bacteria, carbon nanomaterials (e.g., carbon nanotubes, graphene, graphene oxide [GO]) capable of controlling and combating bacterial infections have become some of the most popular, especially GO (Palmieri et al., 2017a, Palmieri et al., 2017b; Grace et al., 2015, Hegab et al., 2016). However, a systematic and comprehensive survey of the literature determined that these carbon nanomaterials cannot generate enough reactive oxygen species (ROS) to fight pathogenic bacteria because during the photocatalytic sterilization process, the requisite electron-hole pairs do not form efficiently enough (Chen et al., 2014a, Tu et al., 2013, Ji et al., 2016).
As graphdiyne (GDY) was first synthesized in 2010 as a new subcategory of 2D graphitic carbon nanomaterials (Li et al., 2010), graphynes, especially GDY, have drawn broad interdisciplinary attention because of their fascinating structures and electronic properties (Li et al., 2014, Zhou et al., 2015). The unique nature of GDY holds great promise for practical applications in batteries, electrics, solar cells, catalysis, detection, and energy storage (Zhou et al., 2015, Zhang et al., 2015, Jia et al., 2017, Kuang et al., 2015, Yang et al., 2013, Li et al., 2017a, Li et al., 2018c, Gao et al., 2019, Xiao et al., 2015). To date, though, the use of GDY and its derivatives in the field of antibacterial research has not been explored or reported. Because GDY has a natural semiconductor band gap and superior electrical properties (Zheng et al., 2018, Inagaki and Kang, 2014), we postulate that it should be capable of serving as a photocatalytic disinfectant to eliminate harmful bacteria, giving rise to a new class of antibacterial nanomaterials. However, the surface of GDY is highly hydrophobic and chemically inert, limiting its interaction with bacteria in an aqueous system and thereby lessening its antibacterial efficacy. As surface oxidation makes 2D nanomaterials more hydrophilic, we propose a new strategy using simple surface oxidation, which modifies the surface of GDY to promote its hydrophilicity and improve its antibacterial activity.
In this work, we report for the first time on the antibacterial behaviors of GDY and graphdiyne oxide (GDYO), focusing on their antibacterial performance and bactericidal mechanisms. Using GDY as a starting material, we synthesize GDYO through surface oxidation with H2O2/H2SO4 as the oxidizing agent, thereby regulating the hydrophilic groups on the GDY surface. To better understand the antibacterial capabilities of GDY and GDYO, we use a series of experiments to explore their antibacterial activity against two models, Escherichia coli and Staphylococcus aureus. Interestingly, GDYO shows higher antibacterial activity than GDY against both models. We attribute this mainly to ROS-dependent oxidative stress, with the prerequisite that the bacteria have direct contact with a dispersed GDYO suspension. On the basis of these findings, we introduce GDYO as a new family of 2D antibacterial nanomaterials that will hopefully open the door for graphdiyne-based nanomaterials in antibacterial and related applications.
Results and Discussion
The GDY employed herein was synthesized on the surface of copper via a cross-coupling reaction according to our previously reported procedure (Li et al., 2010). GDY is a 2D network in which the connecting units consist of a six-membered carbon ring in the center and six carbon triple bonds attached to each of the ring's carbon atoms (Ivanovskii, 2013, Huang et al., 2018). The flat carbon networks contain only sp- and sp2-hybridized carbon atoms with a high π-conjugation (Zhou et al., 2015). As shown in Figure 1A, GDYO was generated by ultrasonic processing on GDY flakes, followed by a modified Hummer's method using a mixture of H2O2/H2SO4 as the oxidizing agent in an ice-water bath. Transmission electron microscopic images reveal that all three products, i.e., GDY (Figures 1B and S1A), ultrasonicated GDY (Figure S2), and GDYO (Figures 1C and S1B), have a thin, sheet-like morphology with occasional folds. A close-up view confirms that all three products are composed of multilayer nanosheets deposited in a high-density tidy orientation, suggesting that the fabrication process that combines ultrasonication and oxidation could render GDYO a sheet-like morphology. Atomic force microscopic images indicate that GDY (Figures 1D and S3A) and GDYO (Figures 1E and S3B) have a thickness of 4–9 nm and 4–11 nm, respectively, further demonstrating that they are made up of multilayer nanosheets with uniform thickness. Dynamic light scattering results reveal that GDY (Figure 1F) and GDYO (Figure 1G) have narrow size (hydrodynamic size) distributions, and their average lateral sizes are ∼410 and ∼370 nm, respectively. Interestingly, GDY shows a larger hydrodynamic size than GDYO, confirming that GDYO has better dispersity in an aqueous solution.
To clarify the differences between the chemical compositions of GDY and GDYO, we performed a scanning transmission electron microscopic (STEM)-mapping test, as seen in Figures 2A (GDY), 2B (GDYO), and S4 (ultrasonicated GDY). The element distributions well match with the sheet-like appearance observable in the STEM images. Interestingly, the signal dots of elemental O (red) corresponded well with those of elemental C (green), on behalf of the oxygen envelope on carbon matrixes for all three products. Compared with GDY, GDYO has a higher oxygen density on the surface, indicating the successful surface oxidation of GDY with H2O2/H2SO4. The presence of carbon and oxygen was further confirmed by the energy-dispersive X-ray spectra (Figure S5), suggesting there are C- and O-containing surface terminations on both GDY and GDYO. A stronger O peak for GDYO than for GDY is also a good evidence of oxidation on the surface of GDY.
Raman spectroscopy and Fourier-transform infrared (FTIR) spectroscopy were also recorded to authenticate the production of GDYO. In Figure 2C, the Raman spectrum of GDY (black curve) presents the D (1,360 cm−1) and G (1,578 cm−1) peaks corresponding to carbon materials, as well as the vibration of acetylenic linkages (C≡C) at around 2,140 cm−1 (Xue et al., 2016, Huang et al., 2015b). After surface oxidation, the as-produced GDYO (red curve in Figure 2C) still retains the D (1,407 cm−1) and G (1,578 cm−1) peaks, and the C≡C (2,143 cm−1) peak is strong, suggesting that the C≡C structures were not destroyed during the oxidation treatment with H2O2/H2SO4. The ratio of the D peak to the G peak is 1.57 for GDY and 1.45 for GDYO, indicating some defects of both GDY and GDYO (Li et al., 2017b, Shi et al., 2018). In Figure 2D, the FTIR spectrum of GDY (black curve) displays three characteristic peaks at 3,423 cm−1, 1,614 cm−1, and 1,266 cm−1, attributable to the -OH stretching vibration, the skeleton vibration of the benzene ring, and the C-O-C stretching vibration, respectively. In addition to these three peaks, the FTIR spectrum of GDYO (red curve) shows a peak for the C=O stretching vibration at 1,723 cm−1 (Zheng et al., 2018), further suggesting the successful oxidation of the GDY nanosheet using H2O2/H2SO4.
The surface functionalization of GDY via surface oxidation was further confirmed by X-ray photoelectron spectroscopy (XPS) (Figures 2E, 2F, and S6). Figure S6 shows two characteristic peaks corresponding to C 1s and O 1s at 284.8 and 531.2 eV (Zhang et al., 2016, Li et al., 2017c), respectively, for both GDY and GDYO. The intensity of the O 1s signal rises noticeably after GDY has been oxidized by H2O2/H2SO4, indicating that the oxygen loading is higher on GDYO than on GDY. When the C 1s spectra is deconvoluted (Figures 2E and 2F), both GDY and GDYO show four separate binding energy peaks at 284.5, 285.5, 286.4, and 289.0 eV, corresponding to C-C sp2, C-C sp, C-O, and C=O, respectively (Li et al., 2016, Jin et al., 2016). Significantly, the intensities of the C-O and C=O peaks in GDYO are higher than those in GDY, indicating more oxygen has been loaded on GDYO than on GDY. The molar percentages (mol %) presented in Table 1 show that those of C-O and C=O in the C 1s peaks increased from 11.21 and 2.79 for GDY to 21.70 and 12.50 for GDYO. Then we measured the zeta potential of GDY and GDYO. As shown in Figure S7, both GDY and GDYO show negative zeta potentials owing to their negatively charged groups on surface, such as -OH, -COOH, C-O-C, etc. Interestingly, GDYO shows a more negative value (−40.89 ± 1.2 mV) than GDY (−35.65 ± 1.0 mV), indicating that GDYO contains more negatively charged groups on surface than GDY.
Table 1.
XPS Peak | Binding Energy (eV) | Mole Percentage (mol %) |
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GDYa | GDYOb | ||
C-C sp2 | 284.5 | 56.02 ± 8.82 | 39.50 ± 8.35 |
C-C sp | 285.5 | 29.98 ± 4.62 | 26.30 ± 5.02 |
C-O | 286.4 | 11.21 ± 3.83 | 21.70 ± 8.18 |
C=O | 289.0 | 2.79 ± 0.37 | 12.50 ± 5.20 |
Graphdiyne.
Graphdiyneoxide.
To the best of our knowledge, the bactericidal effectiveness of graphdiyne-based nanomaterials has not been reported in the literature, although many other carbon nanomaterials, e.g., fullerenes, carbon nanotubes, and graphene derivatives, have been examined and are regarded as good antibacterial nanomaterials (Li et al., 2015, Xin et al., 2018). For the first time, we investigated the antibacterial activity of GDY and GDYO against representative bacteria, using the colony-counting method. When E. coli, a common gram-negative bacteria, was used at a density of 105 colony-forming unit (CFU)·mL−1 as a representative model of pathogenic bacteria (in Figures 3A and S8A), GDYO displayed antibacterial activity but GDY showed very low or no bactericidal ability. After 120 min of incubation, the survival rate of E. coli in the presence of GDYO was 40% in the dark and 2% under visible light irradiation, compared with survival rates exceeding 90% in the presence of GDY, whether in the dark or under visible light irradiation. This difference between GDY and GDYO indicates that surface oxidation grants inert GDY greatly enhanced antibacterial capability, and the increased effectiveness under visible light implies that light has a catalytic effect on the antibacterial action.
The antibacterial property of GDYO was further confirmed by selecting another pathogenic bacteria, S. aureus, a common Gram-positive bacteria. We compared the corresponding results to those with E. coli. Under visible light irradiation (Figures 3B and S8B), E. coli showed 20% survival in the presence of GDYO, compared with no survival by S. aureus under the same conditions, confirming that GDYO is more effective against S. aureus than against E. coli under visible light irradiation. In the dark, a 90% survival rate for E. coli versus 0% survival for S. aureus further demonstrates the greater inhibition impact of GDYO on S. aureus. Generally, whether in the dark or under visible light irradiation, GDYO exhibits stronger antibacterial activity against S. aureus than against E. coli. The differences in cell structure between Gram-positive and Gram-negative bacteria make S. aureus more vulnerable to GDYO than E. coli (Wu et al., 2013, Liu et al., 2009).
The above-mentioned examinations confirmed several factors that influence the antibacterial activity of GDYO, i.e., surface oxidation, visible light irradiation, and bacterial species. We then examined the time-dependent antibacterial action of GDYO via an antibacterial kinetic test. Two bacterial strains (105 CFU·mL−1) were incubated with GDYO dispersion (3.0 mg·mL−1) for different incubation periods, with GDY as the comparative control. To further confirm the impact of visible light on antibacterial efficiency, the kinetic tests were performed in dark and under visible light. The results with E. coli are shown in Figure 3C. The GDYO dispersion exhibited high antibacterial activity both in the dark and under visible light irradiation, whereas the GDY dispersion had almost no bactericidal capacity under either condition during the aging time. Figure 3D presents similar results for S. aureus in the presence of GDYO. As can be observed from both panels (Figures 3C and 3D), the survival rate of the two strains significantly decreased as the contact time was extended, indicating the time dependence of GDYO's antibacterial activity.
As graphdiyne possesses similar structure to graphene, antibacterial activities of GDY and GDYO were compared with those of graphene (GN, Figures S9A and S9B) and GO (Figures S9C and S9D). Figure 3E shows that owing to their sharp edges that have cutting capability, GN and GO have higher antibacterial activities than GDY and GDYO, whether in the dark or under visible light irradiation. Considering that the interaction of graphdiyne-bacteria examined in saline suspension is not very realistic in any kind of application, antibacterial activities of GDY and GDYO were examined in different media (such as water, NaCl 0.9 wt. %, and CaCl2 10 mM) and at different pH values (such as 5.3, 7.3, and 9.3). Obviously, GDY and GDYO show similar antibacterial activity in water, NaCl, and CaCl2 solution (Figure 3F), indicating that the addition of Na+ or Ca2+ cannot drive a significant change in the activities of GDY and GDYO. Compared with ion impact, the impact of pH value on antibacterial action of GDY and GDYO is more remarkable. Compared with those at pH 7.3 and pH 5.3, GDY and GDYO endow the highest activity at pH 9.3 (Figure 3G), suggesting that the antibacterial action of GDY and GDYO is pH dependent.
To understand how GDYO deactivates bacteria, we examined bacterial morphology and membrane integrity in the presence of GDYO, using GDY as the comparative material. Figures 4A, 4D, 4G, 4J, and S10 show that bacterial cells incubated with saline solution remained viable and sustained no observable membrane damage or cell death, displaying a smooth and intact surface. Interestingly, observation of GDY-treated bacteria (Figures 4B, 4E, 4H, and 4K) and GDYO-treated bacteria (Figures 4C, 4F, 4I, and 4L) revealed obvious differences between their surfaces. Both E. coli and S. aureus had relatively smooth surfaces after treatment with GDY, whereas the surfaces of the GDYO-treated bacteria were densely littered with debris, suggesting that GDYO had a destructive effect on the bacteria but GDY did not. We also used STEM-mapping tests to monitor the elemental signals of phosphorus on the bacteria after they were incubated with GDYO; the bacterial cell membrane contains elemental phosphorus, whose distribution might, to some extent, indicate the cell membrane's morphology. The yellow dots in Figure S11 show that the distributions of phosphorus matches well with the corresponding profiles in the STEM images for both E. coli and S. aureus, further implying the morphological changes induced in the bacteria by GDYO.
Some mechanisms have been proposed to explain the antibacterial action of carbon-based nanomaterials, including oxidative stress, interruption of intracellular metabolic routes, and rupture of cell membranes, of which oxidative stress has been regarded as the most likely mechanism for carbon-based nanomaterials including fullerene, carbon nanotubes, and graphene derivatives (Zou et al., 2016). As GDY is a new class of carbon-based nanomaterials, we speculate that oxidative stress might be responsible for the observed antibacterial behavior of GDYO. It is well known that oxidative stress can follow an ROS-dependent or ROS-independent path (Zou et al., 2016). To clarify which paths are followed in terms of GDY and GDYO, we first examined the generation of ROS in the presence of GDY or GDYO using electron spin resonance (ESR) spectroscopy combined with photometric analysis. In addition, as many carbon-based nanomaterials are capable of producing ROS through visible light-dependent reactions and thereby can cause ROS-dependent oxidative antibacterial effects (Chen et al., 2014b), we also tested ROS generation in the presence or absence of visible light irradiation. When ESR tests were performed (Figure 5), neither GDY nor GDYO produced ROS in the dark, but visible light irradiation induced both to generate four ROS species, i.e., hydroxyl radical ⋅OH, superoxide anion radical ⋅O2−, and singlet oxygen 1O2, and hydrogen peroxide H2O2. We conclude that the production of ROS relies mainly on light irradiation rather than the surface structure of graphdiyne. Next, we also used photometric analysis to study the generation of H2O2. Serving similar as pristine H2O2 (Figures S12A and S12B), both GDY and GDYO were able to degrade p-hydroxyphenylacetic acid under visible light irradiation (Nosaka and Nosaka, 2017), indicating the formation of H2O2 in the presence of GDY and GDYO after visible light irradiation. In addition, as shown in Figures 5 and S12C, the generation of ROS is enhanced with both GDY and GDYO when the light exposure time is extended, underscoring the dominant role of visible light irradiation in regulating the generation of ROS, and the time dependence of ROS production.
To understand the active species in the antibacterial process of GDYO, we conducted a scavenging experiment (Chen et al., 2011). using isopropanol alcohol (IPA) to scavenge for ⋅OH (Li et al., 2018d), 4-hydroxy-2,2,6,6-tetramethylpiperidinyloxy (TEMPOL) for ⋅O2− (Zhao et al., 2018), sodium azide (NaN3) for 1O2 (Nosaka and Nosaka, 2017, Wang et al., 2018), and ethylenediaminetetraacetic acid ferric sodium salt (Fe(II)) for H2O2 (Zhang et al., 2018). Before the experiment, we made sure that all the scavengers (the red curves in Figures 6A–6D) in the concentrations to be used had no (or quite low) bactericidal activity. As shown in Figure 6A, the addition of IPA into the GDYO + Light system scarcely increased bacterial survival, indicating that little ⋅OH is involved in GDYO's antibacterial activity. Bacterial survival increased a bit when TEMPOL (Figure 6B) or Fe (II) (Figure 6D) was added into the GDYO + Light system, demonstrating that ⋅O2− and H2O2 make a certain contribution to GDYO's antibacterial activity. Interestingly, the GDYO + Light system showed no antibacterial activity in the presence of NaN3 (Figure 6C), illustrating that 1O2 is the main factor regulating GDYO's antibacterial activity. Hence, in terms of their contribution to GDYO's ROS-dependent antibacterial action (Figure 6E), 1O2 plays a major role, followed by H2O2 and ⋅O2−, and ⋅OH makes the least contribution.
Through the combination of antibacterial results (Figure 3) and ROS analysis (Figure 5), we confirm that GDY has no (or quite low) antibacterial function, whether or not it produces ROS, whereas GDYO kills bacteria in the absence and presence of ROS. Accordingly, we envision that in addition to ROS, GDYO's toxicity toward bacteria may be attributable to a second factor. It has been reported that 2D carbon nanomaterials (e.g., GO nanosheets) can display a blade-like action that destroys the bacterial membrane, presenting a contact-based mechanism (Geng et al., 2017, Akhavan and Ghaderi, 2010). To elucidate the exact role that direct contact plays in GDYO's antibacterial efficiency, we prepared a dried GDYO membrane on the filter (Li et al., 2019), and then E. coli suspension was dropped evenly on the surface of GDYO membrane. After a direct contact for 30 min in the dark, E. coli on the GDYO membrane was washed with ultrapure water, and the survival of E. coli in water was detected using the colony-counting method. As a result, GDYO shows certain bacteria-killing ability by contact (Figure S13), indicating that GDYO possess antibacterial activity even without oxidative stress induced by ROS. Accordingly, one can be sure that GDYO kills bacteria in the dark via the direct contact between bacteria and GDYO.
For antibacterial materials that require contact to be effective, good dispersion in the bacterial suspension is required. So we examined the dispersion behaviors of GDY and GDYO in water. Representative photographs of GDY and GDYO dispersions (concentration: 1.0 mg·mL−1) are shown in Figure 7A. Clearly, GDY and GDYO look different because of their distinct structural and physicochemical properties. A black suspension is visible in the GDY dispersion after sonication treatment, and most of the GDY precipitated after standing for ≥12 h. The GDYO dispersion obtained by sonication treatment was opaque yellow, and even after standing for 36 h, it remained stable as a homogeneous yellow dispersion with only a small portion of the GDYO precipitating out. Then, turbidity and light scattering of GDY and GDYO dispersion were measured. Figure 7B shows the turbidity of GDY and GDYO as a function of aging time. Obviously, the turbidity of GDYO is higher than that of GDY, demonstrating that GDYO is more stable than GDY in water. We ascribe the good dispersion of GDYO in water to the rich hydrophilic functional groups on the surface of GDYO nanosheets, such as the carboxyl, hydroxyl, and epoxy groups that the FTIR and XPS spectra demonstrated to be present (Figures 2D–2F). The better dispersion of GDYO is consistent with the better antibacterial performance of GDYO compared with GDY (Figure 3), indicating that the higher the dispersion, the more active it will be (Liu et al., 2011). To further understand the dispersion stability of GDY and GDYO, light scattering was performed (Figure 7C). Compared with other carbon materials (Goscianska et al., 2019), GDY and GDYO show high transmission level, indicating that neither GDY nor GDYO is stable enough in static water.
To further confirm the dispersion-dependent antibacterial action of GDYO when it exists in a dispersed form, we then carried out an inhibition zone test in which GDY was employed as the comparative material. As shown in Figure 7D, under visible light irradiation, both GDY and GDYO had inhibition zones with an average diameter of 32.1 and 41.7 mm, respectively, whereas they had no bacteriostatic areas under dark conditions. This is another convincing proof of the importance of visible light irradiation for the antibacterial behavior of GDY and GDYO. With respect to the antibacterial mechanism, the appearance of inhibition zones around GDY and GDYO indicate that at least some of the active components were released from the specimen disk and extended outward, thereby contributing to the antibacterial effect. Such a release-based antibacterial action is especially noticeable from the opaque yellow ring around the GDYO sample under visible light irradiation. When GDY and GDYO are compared, the latter had a larger inhibition zone, which could be explained by GDYO's better dispersion. Strangely, although GDY showed almost no antibacterial activity in the colony-counting test (Figure 3), it showed an obvious inhibition effect under visible light irradiation (Figure 7D). This could be because GDY is more easily exposed to visible light during the inhibition zone test than in the colony-counting test, so its antibacterial behavior can be amplified in the former test.
Based on the above research, GDYO has the following three antibacterial mechanisms: (1) initial good dispersion in the bacterial system, (2) direct contact with the bacteria, and (3) ROS-dependent oxidation stress. Figure 7E presents a diagram of these proposed mechanisms. According to our step-by-step verification of the antibacterial mechanisms, ROS-dependent oxidation stress (especially under visible light irradiation) is the most lethal, but good dispersion and direct contact with the bacteria are prerequisites for the ROS mechanism. We believe that a single antibacterial mechanism may not be sufficient for GDYO to be an effective bactericidal material, and that the three factors affect each other. Owing to its superior antibacterial activity induced by these different mechanisms, GDYO is more potent against the two tested bacteria than GDY, suggesting that surface oxidation grants inert GDY superior antibacterial capability.
Conclusions
We synthesized GDYO from GDY by a simple surface oxidation process, and the subsequent characterizations confirmed that GDYO has a nanosheet-like morphology and abundant carboxyl, hydroxyl, and epoxy functional groups on its surface. Assessment of GDYO's antibacterial activity as an aqueous dispersion showed that it was quite active toward both E. coli and S. aureus, whereas GDY had little to no antibacterial activity under the same conditions. In addition, the antibacterial activities of GDYO are dependent on visible light, bacterial species, and time. We confirmed that GDYO has a combined antibacterial mechanism that includes dispersion in the bacterial system, direct contact with the bacteria, and ROS-dependent oxidation stress. All these efforts shed light on the bactericidal actions of graphdiyne-based nanomaterials and support their future use in antibacterial applications.
Limitations of Study
Although it is demonstrated that the hydrophilic functional groups on the surface of GDYO nanosheets enable better dispersion in water and allow higher antibacterial efficiency, the relationships between the functional groups and the antibacterial efficiency have not been fully discussed. We believe that further optimizing the functional groups on the surfaces of GDYO nanosheets may further promote the antibacterial activity.
Methods
All methods can be found in the accompanying Transparent Methods supplemental file.
Acknowledgments
This research was supported by the National Natural Science Foundation of China (21304044, 51663019, 21672222, and 21790051), the Natural Science Foundation of Inner Mongolia Autonomous Region (2015MS0520), the State Key Laboratory of Medicinal Chemical Biology (201603006 and 2018051), the State Key Laboratory of Polymer Physics and Chemistry (2018-08), and the Program of Higher-Level Talents of Inner Mongolia University (30105-125136). Y.-W.Y. acknowledges Jilin Province-University Cooperative Construction Project-Special Funds for New Materials (SXGJSF2017-3) and Jilin University Talents Cultivation Program for financial support.
Author Contributions
Y.L, Y.-W.Y., A.D., and Y.L. conceived and designed the project. Y.Z. and W.L. performed the experiments under the guidance of Y.L, Y.-W.Y., A.D., and Y.L., including materials synthesis, characterization, antibacterial test, and mechanism verification. All authors discussed the data and commented on the manuscript. Y.Z. and W.L. wrote the paper, and Y.L, Y.-W.Y., A.D., and Y.L. revised the manuscript.
Declaration of Interests
The authors declare no competing financial interests.
Published: September 27, 2019
Footnotes
Supplemental Information can be found online at https://doi.org/10.1016/j.isci.2019.08.019.
Contributor Information
Yongjun Li, Email: liyj@iccas.ac.cn.
Ying-Wei Yang, Email: ywyang@jlu.edu.cn.
Alideertu Dong, Email: dongali@imu.edu.cn.
Supplemental Information
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