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. 2019 Sep 3;10(51):5332–5348. doi: 10.18632/oncotarget.27152

Figure 1. Temperature-dependent effect of SV40T expression on ciPTEC-OAT1 proliferation and apoptosis-sensitivity.

Figure 1

(A) Western blot analysis of SV40T levels in ciPTEC-OAT1 cultured at the permissive (33° C) temperature and the non-permissive (37° C) temperature for 1 day, 7 days or 7 days followed by a 4 h incubation at 33° C (switch). Intensity of the bands was normalized to GAPDH and quantification is depicted in the bar graph. Human kidney tissue protein sample served as control. Representative histograms and analysis of cell cycle distribution of ciPTEC-OAT1 cultured at (B) subconfluent and (C) confluent levels at 33° C and 37° C for 1 day, 7 days or 7 days followed by 4 h at 33° C (switch). (D) Cell viability analysis and (E) caspase-3/7 expression in ciPTEC-OAT1 cultured at 33° C and 37° C and exposed to increasing concentrations of nutlin-3a for 24 h. All values are expressed as the mean ± SEM of three independent experiments performed in triplicate. * p < 0.05, ** p < 0.01, *** p < 0.001 (unpaired two-tailed Student’s t-test and one-way ANOVA followed by Dunnett’s multiple comparison test).