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. 2007 May 1;5(1):49–59. doi: 10.1111/j.1582-4934.2001.tb00137.x

Detection of human papillomavirus gene sequences in cell lines derived from laryngeal tumors

A Mutiu 1, Irina Alexiu 1, Mihaela Chivu 1, M Petica 1, Gabriela Anton 1, Coralia Bleotu 1, Carmen Diaconu 1, C Popescu 2,3, V Jucu 1, C Cernescu 1,3,✉
PMCID: PMC6737758  PMID: 12067450

Abstract

The role of Human Papillomaviruses (HPV) in laryngeal carcinomas has been studied with conflicting results. To evaluate the etiologic relationship between HPV infection and epithelial malignancy of the larynx we studied five laryngeal carcinoma cell lines obtained from patients undergoing surgery for laryngeal tumors. The paraffin embedded biopsy samples of the original tumor and different passages of the new established cell lines were investigated by PCR with consensus primers specific for HPV DNA. The findings indicate that HPV infection is associated with some larynx carcinomas. The positive association has been enhanced when a method of enrichment of epithelial cells from fresh tumor samples was used. All tumor cells enriched smears were positive for HPV DNA not only by PCR but also by in situ hybridization (ISH). Investigated by PCR, different passages of larynx tumor cell lines maintained expression of HPV DNA. At subsequent passages ISH gives constantly no signals suggesting a minimal amount of viral harbored sequences. In one cell line propagated more than 60 population doublings, the chromosomal frequency distribution shifted from modal number 46 at the 5th passage to 63 at the 60th passage. The mechanisms by which persistent HPV infection maintains continuous cell proliferation were discussed.

Keywords: laryngeal carcinomas, enrichment of epithelial cells, tumor derived cell lines, HPV DNA detection

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