End1/E6E7 cell cultures treated with indicated concentrations of IL-22 for 12 h . Cellular proteins were subjected to Western blot assay using antibodies to STATs (A), ISGs (C) and TJs (E). (B, D, F) Densitometry analysis of relative STATs, ISGs and TJ protein levels compared with β-actin was performed by ImageJ 1.44 software. Data are shown as mean ± SD for three independent experiments (*P < 0.05, **P < 0.01).